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Literature summary for 1.4.1.14 extracted from

  • SLu, Y.; Luo, F.; Yang, M.; Li, X.; Lian, X.
    Suppression of glutamate synthase genes significantly affects carbon and nitrogen metabolism in rice (Oryza sativa L.) (2011), Sci. China Life Sci., 54, 651-663.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
NADH-GOGAT1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, relative real-time RT-PCR expression analysis Oryza sativa Japonica Group
NADH-GOGAT2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, relative real-time RT-PCR expression analysis Oryza sativa Japonica Group

Protein Variants

Protein Variants Comment Organism
additional information suppression of the enzyme leads to significantly increased 2-oxoglutarate levels in leaves in most co-suppressed lines compared with the wild-type, in the leaf, the 2-oxoglutarate precursor, isocitrate, shows the same trend. The contents of oxaloacetate, malate, and pyruvate are also increased in some, but not all, co-suppressed lines. The mutants show altered chlorophyll contents, phenotypes, overview Oryza sativa Japonica Group

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Oryza sativa Japonica Group 9507
-
plastid NADH-GOGAT is primarily located in plastids of non-photosynthetic tissues such as roots Oryza sativa Japonica Group 9536
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 L-glutamate + NAD+ Oryza sativa Japonica Group
-
L-glutamine + 2-oxoglutarate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Oryza sativa Japonica Group B9FLJ1 isozyme GOGAT2; isozyme GOGAT1
-
Oryza sativa Japonica Group Q0JKD0 isozyme GOGAT1; isozyme GOGAT1
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Oryza sativa Japonica Group
-
root NADH-GOGAT is primarily located in plastids of non-photosynthetic tissues such as roots Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-glutamate + NAD+
-
Oryza sativa Japonica Group L-glutamine + 2-oxoglutarate + NADH + H+
-
r

Synonyms

Synonyms Comment Organism
glutamine 2-oxoglutarate aminotransferase
-
Oryza sativa Japonica Group
NADH-GOGAT
-
Oryza sativa Japonica Group

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Oryza sativa Japonica Group

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Oryza sativa Japonica Group
NADH
-
Oryza sativa Japonica Group