Cloned (Comment) | Organism |
---|---|
NADH-GOGAT1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, relative real-time RT-PCR expression analysis | Oryza sativa Japonica Group |
NADH-GOGAT2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, relative real-time RT-PCR expression analysis | Oryza sativa Japonica Group |
Protein Variants | Comment | Organism |
---|---|---|
additional information | suppression of the enzyme leads to significantly increased 2-oxoglutarate levels in leaves in most co-suppressed lines compared with the wild-type, in the leaf, the 2-oxoglutarate precursor, isocitrate, shows the same trend. The contents of oxaloacetate, malate, and pyruvate are also increased in some, but not all, co-suppressed lines. The mutants show altered chlorophyll contents, phenotypes, overview | Oryza sativa Japonica Group |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Oryza sativa Japonica Group | 9507 | - |
plastid | NADH-GOGAT is primarily located in plastids of non-photosynthetic tissues such as roots | Oryza sativa Japonica Group | 9536 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Oryza sativa Japonica Group |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-glutamate + NAD+ | Oryza sativa Japonica Group | - |
L-glutamine + 2-oxoglutarate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa Japonica Group | B9FLJ1 | isozyme GOGAT2; isozyme GOGAT1 | - |
Oryza sativa Japonica Group | Q0JKD0 | isozyme GOGAT1; isozyme GOGAT1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Oryza sativa Japonica Group | - |
root | NADH-GOGAT is primarily located in plastids of non-photosynthetic tissues such as roots | Oryza sativa Japonica Group | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-glutamate + NAD+ | - |
Oryza sativa Japonica Group | L-glutamine + 2-oxoglutarate + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
glutamine 2-oxoglutarate aminotransferase | - |
Oryza sativa Japonica Group |
NADH-GOGAT | - |
Oryza sativa Japonica Group |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Oryza sativa Japonica Group |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Oryza sativa Japonica Group | |
NADH | - |
Oryza sativa Japonica Group |