Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.1.14 extracted from

  • Singhal, R.K.; Krishnan, I.S.; Dua, R.D.
    Stabilization, purification, and characterization of glutamate synthase from Clostridium pasteurianum (1989), Biochemistry, 28, 7928-7935.
    View publication on PubMed
Search for more literature for 1.4.1.14

General Stability

General Stability Organism
2-oxoglutarate, 2-mercaptoethanol and EDTA stabilize and have a synergystic effect Clostridium pasteurianum
sodium dithionite and glutamine stabilize Clostridium pasteurianum

Inhibitors

Inhibitors Comment Organism Structure
asparagine slight inhibition Clostridium pasteurianum
Co2+ slight inhibition Clostridium pasteurianum
Cu2+ Cu2+ and Fe2+ are most inhibitory metal ions Clostridium pasteurianum
Fe2+ Cu2+ and Fe2+ are most inhibitory metal ions Clostridium pasteurianum
glycine slight inhibition Clostridium pasteurianum
L-glutamate 4 mM, 20% inhibition, product inhibition Clostridium pasteurianum
L-histidine 25 mM, 20-30% inhibition Clostridium pasteurianum
methionine 25 mM L-methionine, 20-30% inhibition Clostridium pasteurianum
Mg2+ slight inhibition Clostridium pasteurianum
additional information not inhibited by Mn2+ and Ca2+ Clostridium pasteurianum
pyruvate slight inhibition, analogue of 2-oxoglutarate Clostridium pasteurianum
serine 25 mM L-serine, 20-30% inhibition Clostridium pasteurianum
Sodium dithionite strong inhibitory effect Clostridium pasteurianum
tryptophan 25 mM L-tryptophan, 20-30% inhibition Clostridium pasteurianum
Zn2+ slight inhibition Clostridium pasteurianum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 NADH
-
 Clostridium pasteurianum
0.16
-
 L-glutamine
-
 Clostridium pasteurianum
0.17
-
 2-oxoglutarate
-
 Clostridium pasteurianum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information absence of flavin and iron Clostridium pasteurianum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17500
-
 2 * 91000 + 2 * 86000 + 2 * 68000 + 2 * 31000 + 2 * 17500, SDS-PAGE Clostridium pasteurianum
31000
-
 2 * 91000 + 2 * 86000 + 2 * 68000 + 2 * 31000 + 2 * 17500, SDS-PAGE Clostridium pasteurianum
68000
-
 2 * 91000 + 2 * 86000 + 2 * 68000 + 2 * 31000 + 2 * 17500, SDS-PAGE Clostridium pasteurianum
86000
-
 2 * 91000 + 2 * 86000 + 2 * 68000 + 2 * 31000 + 2 * 17500, SDS-PAGE Clostridium pasteurianum
91000
-
 2 * 91000 + 2 * 86000 + 2 * 68000 + 2 * 31000 + 2 * 17500, SDS-PAGE Clostridium pasteurianum
590000
-
 gel filtration Clostridium pasteurianum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamine + 2-oxoglutarate + NADH Clostridium pasteurianum regulatory role in biosynthesis pathways involving glutamate, early acceptor of fixed nitrogen glutamate + NAD+
-
 ?
L-glutamine + 2-oxoglutarate + NADH Clostridium pasteurianum ammonia assimilation glutamate + NAD+
-
 ?

Organism

Organism UniProt Comment Textmining
Clostridium pasteurianum
-
 strain ATCC 6013
-

Purification (Commentary)

Purification (Comment) Organism
58fold purification Clostridium pasteurianum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.8
-
-
 Clostridium pasteurianum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 2-oxoglutarate + NADH 1 mol 2-oxoglutarate and 1 mol glutamine are consumed per mol NADH oxidized Clostridium pasteurianum L-glutamate + NAD+
-
 ?
L-glutamine + 2-oxoglutarate + NADH highly specific for its substrates Clostridium pasteurianum L-glutamate + NAD+
-
 ?
L-glutamine + 2-oxoglutarate + NADH specific for NADH Clostridium pasteurianum L-glutamate + NAD+
-
 ?
L-glutamine + 2-oxoglutarate + NADH regulatory role in biosynthesis pathways involving glutamate, early acceptor of fixed nitrogen Clostridium pasteurianum glutamate + NAD+
-
 ?
L-glutamine + 2-oxoglutarate + NADH ammonia assimilation Clostridium pasteurianum glutamate + NAD+
-
 ?
additional information no activity with L-asparagine, NH4Cl, pyruvate, oxaloacetate or NADPH Clostridium pasteurianum ?
-
 ?
additional information no activity with albizziin, 6-diazo-5-oxo-L-norvaline, azaserine, methionine sulfoximine, FADH or FMNH Clostridium pasteurianum ?
-
 ?

Subunits

Subunits Comment Organism
decamer 2 * 91000 + 2 * 86000 + 2 * 68000 + 2 * 31000 + 2 * 17500, SDS-PAGE Clostridium pasteurianum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Clostridium pasteurianum
50
-
-
 Clostridium pasteurianum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 enzyme loses activity after incubation for 10 min Clostridium pasteurianum
50
-
 half-life: 10 min, presence of 2-oxoglutarate and L-glutamine stabilizes against thermal denaturation, whereas preincubation with NADH or L-glutamate causes more heat sensitivity Clostridium pasteurianum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 broad pH profile with optimum at pH 7.2 Clostridium pasteurianum

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADPH Clostridium pasteurianum
additional information absence of flavin and iron Clostridium pasteurianum
NADH specific for NADH as reductant Clostridium pasteurianum