Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.1.13 extracted from

  • Vanoni, M.A.; Verzotti, E.; Zanetti, G.; Curti, B.
    Properties of the recombinant beta subunit of glutamate synthase (1996), Eur. J. Biochem., 236, 937-946.
    View publication on PubMed
Search for more literature for 1.4.1.13

Cloned(Commentary)

Cloned (Comment) Organism
production of the beta subunit in Escherichia coli Azospirillum brasilense

Inhibitors

Inhibitors Comment Organism Structure
2'-phosphoadenosine-5'-diphospho-5'-beta-D-ribose for the NADPH: acceptor oxidoreductase activity of the beta subunit of the enzyme, uncompetitive inhibition with ferricyanide or iodonitrotetrazolium as substrate, competitive inhibition with NADPH as substrate Azospirillum brasilense

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0035
-
 NADPH of NADPH: acceptor oxidoreductase activity of the beta subunit of the enzyme, acceptor: iodonitrotetrazolium Azospirillum brasilense
0.0098
-
 NADPH of NADPH: acceptor oxidoreductase activity of the beta subunit of the enzyme, acceptor: menadione Azospirillum brasilense
0.0118
-
 NADPH of NADPH: acceptor oxidoreductase activity of the beta subunit of the enzyme, acceptor: ferricyanide Azospirillum brasilense
0.035
-
 menadione of NADPH: acceptor oxidoreductase activity of the beta subunit of the enzyme Azospirillum brasilense
0.05
-
 iodonitrotetrazolium of NADPH: acceptor oxidoreductase activity of the beta subunit of the enzyme Azospirillum brasilense
0.14
-
 ferricyanide of NADPH: acceptor oxidoreductase activity of the beta subunit of the enzyme Azospirillum brasilense

Metals/Ions

Metals/Ions Comment Organism Structure
Iron iron-sulfur protein Azospirillum brasilense

Organism

Organism UniProt Comment Textmining
Azospirillum brasilense
-
-
-

Purification (Commentary)

Purification (Comment) Organism
of the recombinant enzyme beta subunit, using ammonium sulfate fractionation, affinity chromatography on Reactive Red, ultrafiltration and column chromatography on Ultrogel AcA 54 Azospirillum brasilense

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ferricyanide + NADPH + H+
-
 Azospirillum brasilense ferrocyanide + NADP+
-
 r
iodonitrotetrazolium + NADPH + H+
-
 Azospirillum brasilense reduced iodonitrotetrazolium + NADP+
-
 r
L-glutamine + 2-oxoglutarate + NADPH + H+
-
 Azospirillum brasilense L-glutamate + NADP+
-
 ?
menadione + NADPH + H+
-
 Azospirillum brasilense menadiol + NADP+
-
 r
additional information the enzyme beta subunit is devoid of glutamate synthase activity in either direction at both pH 7.5 and 9.5, but it can oxidize NADPH and transfer electrons to synthetic electron acceptors like iodonitrotetrazolium, ferricyanide, menadione, dichloroindophenol, the beta subunit is highly specific toward NADPH, the rate of oxidation of NADH in the presence of electron acceptors is less than 5% of that measured with NADPH Azospirillum brasilense ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
18.3
-
 NADPH of NADPH:acceptor oxidoreductase activity of the beta subunit of the enzyme, co-substrate: iodonitrotetrazolium, inhibitor: 2'-phosphoadenosine-5'-diphospho-5'-beta-D-ribose Azospirillum brasilense
20
-
 iodonitrotetrazolium of NADPH:acceptor oxidoreductase activity of the beta subunit of the enzyme, co-substrate: NADPH, inhibitor: 2'-phosphoadenosine-5'-diphospho-5'-beta-D-ribose Azospirillum brasilense
25.3
-
 menadione of NADPH:acceptor oxidoreductase acitivity of the beta subunit of the enzyme Azospirillum brasilense
30.8
-
 NADPH of NADPH:acceptor oxidoreductase activity of the beta subunit of the enzyme, co-substrate: ferricyanide, inhibitor: 2'-phosphoadenosine-5'-diphospho-5'-beta-D-ribose Azospirillum brasilense
32
-
 iodonitrotetrazolium of NADPH:acceptor oxidoreductase acitivity of the beta subunit of the enzyme Azospirillum brasilense
39.5
-
 ferricyanide of NADPH:acceptor oxidoreductase activity of the beta subunit of the enzyme, co-substrate: NADPH, inhibitor: 2'-phosphoadenosine-5'-diphospho-5'beta-D-ribose Azospirillum brasilense
41.9
-
 ferricyanide of NADPH:acceptor oxidoreductase acitivity of the beta subunit of the enzyme Azospirillum brasilense

Cofactor

Cofactor Comment Organism Structure
flavin flavoenzyme Azospirillum brasilense
flavin the beta subunit contains the binding site for FAD, 0.83 mol FAD per mol beta subunit Azospirillum brasilense
additional information the enzyme contains three distinct ion-sulfur centers per alphabeta protomer Azospirillum brasilense
NADPH the beta subunit contains the NADPH binding site of the enzyme Azospirillum brasilense

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.021
-
 2'-phosphoadenosine-5'-diphospho-5'-beta-D-ribose for the NADPH:acceptor oxidoreductase activity of the beta subunit of the enzyme, substrate: NADPH, cosubstrate: ferricyanide Azospirillum brasilense
0.08
-
 2'-phosphoadenosine-5'-diphospho-5'-beta-D-ribose for the NADPH:acceptor oxidoreductase activity of the beta subunit of the enzyme, substrate: NADPH, cosubstrate: iodonitrotetrazolium Azospirillum brasilense
0.18
-
 2'-phosphoadenosine-5'-diphospho-5'-beta-D-ribose for the NADPH:acceptor oxidoreductase activity of the beta subunit of the enzyme, substrate: ferricyanide, cosubstrate: NADPH Azospirillum brasilense
0.495
-
 2'-phosphoadenosine-5'-diphospho-5'-beta-D-ribose for the NADPH:acceptor oxidoreductase activity of the beta subunit of the enzyme, substrate: iodonitrotetrazolium, cosubstrate: NADPH Azospirillum brasilense