Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-2-Amino-4-oxo-5-chloropentanoic acid | selective inhibitor of glutamine-dependent activity | Escherichia coli | |
L-2-Amino-4-oxo-5-chloropentanoic acid | selective inhibitor of glutamine-dependent activity | Klebsiella aerogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
NADPH | - |
Klebsiella aerogenes | |
0.3 | - |
L-glutamine | - |
Klebsiella aerogenes | |
0.3 | - |
alpha-ketoglutarate | - |
Klebsiella aerogenes | |
0.5 | 2 | NH4Cl | - |
Klebsiella aerogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51500 | - |
x * 175000 + x * 51500, SDS-PAGE | Klebsiella aerogenes |
175000 | - |
x * 175000 + x * 51500, SDS-PAGE | Klebsiella aerogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Klebsiella aerogenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using sonication, heat treatment, protamine sulfate precipitation, ammonium sulfate precipitation, gel filtration on G-50, column chromatography on DEAE-Sephadex and gel filtration on Sephadex G-200 and Sepharose 6B | Klebsiella aerogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
additional information | - |
- |
Klebsiella aerogenes |
13.33 | - |
- |
Escherichia coli |
20.83 | - |
- |
Klebsiella aerogenes |
Storage Stability | Organism |
---|---|
4°C, 200 mM potassium phosphate buffer, pH 7.6, 0.5 mM Na2EDTA | Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2-oxoglutarate + NADPH + H+ | in the reverse reaction ammonia can act instead of glutamine, but more slowly | Escherichia coli | L-glutamate + NADP+ | - |
? | |
L-glutamine + 2-oxoglutarate + NADPH + H+ | in the reverse reaction ammonia can act instead of glutamine, but more slowly | Klebsiella aerogenes | L-glutamate + NADP+ | - |
? | |
additional information | - |
Escherichia coli | ? | - |
? | |
additional information | glutamine binding site of the enzyme is located on the heavy subunit of the enzyme, preparations of the enzyme that lack flavins or the flavins and iron sulfide catalyze NH3-dependent reaction but not glutamine-dependent reaction | Klebsiella aerogenes | ? | - |
? | |
NH3 + 2-oxoglutarate + NADPH + H+ | - |
Escherichia coli | L-glutamate + NADP+ | - |
? | |
NH3 + 2-oxoglutarate + NADPH + H+ | - |
Klebsiella aerogenes | L-glutamate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 175000 + x * 51500, SDS-PAGE | Klebsiella aerogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
glutamine-dependent activity | Klebsiella aerogenes |
8 | - |
glutamine-dependent activity | Klebsiella aerogenes |
9 | - |
ammonia-dependent activity | Klebsiella aerogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | no activity with NADH | Escherichia coli | |
NADH | no activity with NADH | Klebsiella aerogenes | |
NADPH | - |
Escherichia coli | |
NADPH | - |
Klebsiella aerogenes |