Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-Bromo-2-oxoglutarate | - |
Escherichia coli | |
4-Iodoacetamidosalicylate | - |
Escherichia coli | |
Bromopyruvate | - |
Escherichia coli | |
iodoacetamide | selective inactivation of glutamine-dependent activity | Escherichia coli | |
L-2-Amino-4-oxo-5-chloropentanoic acid | selective inhibitor of glutamine-dependent activity | Escherichia coli | |
N-ethylmaleimide | - |
Escherichia coli | |
NADPH | inactivation of glutamine-dependent activity, 50% inactivation at 0.36 mM in 10 min | Escherichia coli | |
p-chloromercuribenzoate | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using streptomycin sulfate treatment, ammonium sulfate fractionation, heat treatment, agarose gel filtration and DEAE-cellulose column chromatography | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14.3 | - |
native enzyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2-oxoglutarate + NADPH + H+ | in the reverse reaction ammonia can act instead of glutamine, but more slowly | Escherichia coli | L-glutamate + NADP+ | - |
? | |
NH3 + 2-oxoglutarate + NADPH + H+ | the specific activity of native enzyme using NH3 varies between 5% and 7% of the glutamine-dependent activity | Escherichia coli | L-glutamate + NADP+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
L-2-Amino-4-oxo-5-chloropentanoic acid | - |
Escherichia coli |