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Literature summary for 1.4.1.13 extracted from

  • Tachiki, T.; Sung, H.C.; Wakisaka, S.; Tochikura, T.
    Purification and some properties of glutamate synthase from Gluconobacter subodydans on glutamate as a nitrogen source (1983), J. Ferment. Technol., 61, 179-184.
No PubMed abstract available

General Stability

General Stability Organism
alpha-ketoglutarate stabilizes Gluconobacter oxydans
EDTA, stabilizes Gluconobacter oxydans
glutamine stabilizes Gluconobacter oxydans

Inhibitors

Inhibitors Comment Organism Structure
alpha-aminobutyrate
-
Gluconobacter oxydans
D-glutamate
-
Gluconobacter oxydans
D-glutamine
-
Gluconobacter oxydans
DL-methionine DL-sulfoximine potent inhibitor Gluconobacter oxydans
DL-Theanine
-
Gluconobacter oxydans
hydroxylamine
-
Gluconobacter oxydans
L-alanine
-
Gluconobacter oxydans
L-aspartate
-
Gluconobacter oxydans
L-histidine
-
Gluconobacter oxydans
L-homoserine
-
Gluconobacter oxydans
L-leucine
-
Gluconobacter oxydans
L-methionine
-
Gluconobacter oxydans
lysine
-
Gluconobacter oxydans
NADH partially inhibits Gluconobacter oxydans
NADP+ potent inhibitor Gluconobacter oxydans
oxaloacetate
-
Gluconobacter oxydans
putrescine
-
Gluconobacter oxydans
pyruvate
-
Gluconobacter oxydans
valine
-
Gluconobacter oxydans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0086
-
alpha-ketoglutarate
-
Gluconobacter oxydans
0.014
-
NADPH
-
Gluconobacter oxydans
0.59
-
L-glutamine
-
Gluconobacter oxydans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
500000
-
gel filtration Gluconobacter oxydans

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using ammonium sulfate fractionation, coulmn chromatography on DEAE-cellulose, hydroxyapatite, Sepharose 6B and Sephadex G-200 Gluconobacter oxydans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.72
-
-
Gluconobacter oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 2-oxoglutarate + NADPH + H+ highly specific Gluconobacter oxydans L-glutamate + NADP+
-
?
additional information amino acids, amines and ammonium chloride do not substitute for L-glutamine, other alpha-keto acids including oxalacetate, pyruvate, glyoxylate and alpha-ketobutyrate do not support the activity Gluconobacter oxydans ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
the enzyme retains its full activity after heating for 10 min in 0.1 M potassium phosphate buffer, pH 6.8 Gluconobacter oxydans
55
-
10 min, in 0.1 M potassium phosphate buffer, pH 6.8, 70-80% loss of activity Gluconobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Gluconobacter oxydans

pH Range

pH Minimum pH Maximum Comment Organism
8 9
-
Gluconobacter oxydans

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Gluconobacter oxydans

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.02
-
DL-methionine DL-sulfoximine competitive inhibitor with respect to alpha-keto-glutarate Gluconobacter oxydans
0.07
-
NADP competitive inhibition with NADPH Gluconobacter oxydans
0.12
-
NADP mixed-type inhibition with alpha-ketoglutarate Gluconobacter oxydans
0.14
-
DL-methionine DL-sulfoximine competitive inhibitor with respect to glutamine Gluconobacter oxydans
0.3
-
NADP uncompetitive inhibition with respect to glutamine Gluconobacter oxydans
0.48
-
methionine competitive inhibitor with respect to alpha-keto-glutarate Gluconobacter oxydans
0.76
-
DL-methionine DL-sulfoximine noncompetitive inhibitor with respect to NADPH Gluconobacter oxydans
3
-
methionine competitive inhibitor with respect to glutamine Gluconobacter oxydans
5.5
-
methionine noncompetitive inhibitor with respect to NADPH Gluconobacter oxydans