General Stability | Organism |
---|---|
alpha-ketoglutarate stabilizes | Gluconobacter oxydans |
EDTA, stabilizes | Gluconobacter oxydans |
glutamine stabilizes | Gluconobacter oxydans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-aminobutyrate | - |
Gluconobacter oxydans | |
D-glutamate | - |
Gluconobacter oxydans | |
D-glutamine | - |
Gluconobacter oxydans | |
DL-methionine DL-sulfoximine | potent inhibitor | Gluconobacter oxydans | |
DL-Theanine | - |
Gluconobacter oxydans | |
hydroxylamine | - |
Gluconobacter oxydans | |
L-alanine | - |
Gluconobacter oxydans | |
L-aspartate | - |
Gluconobacter oxydans | |
L-histidine | - |
Gluconobacter oxydans | |
L-homoserine | - |
Gluconobacter oxydans | |
L-leucine | - |
Gluconobacter oxydans | |
L-methionine | - |
Gluconobacter oxydans | |
lysine | - |
Gluconobacter oxydans | |
NADH | partially inhibits | Gluconobacter oxydans | |
NADP+ | potent inhibitor | Gluconobacter oxydans | |
oxaloacetate | - |
Gluconobacter oxydans | |
putrescine | - |
Gluconobacter oxydans | |
pyruvate | - |
Gluconobacter oxydans | |
valine | - |
Gluconobacter oxydans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0086 | - |
alpha-ketoglutarate | - |
Gluconobacter oxydans | |
0.014 | - |
NADPH | - |
Gluconobacter oxydans | |
0.59 | - |
L-glutamine | - |
Gluconobacter oxydans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
500000 | - |
gel filtration | Gluconobacter oxydans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using ammonium sulfate fractionation, coulmn chromatography on DEAE-cellulose, hydroxyapatite, Sepharose 6B and Sephadex G-200 | Gluconobacter oxydans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.72 | - |
- |
Gluconobacter oxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2-oxoglutarate + NADPH + H+ | highly specific | Gluconobacter oxydans | L-glutamate + NADP+ | - |
? | |
additional information | amino acids, amines and ammonium chloride do not substitute for L-glutamine, other alpha-keto acids including oxalacetate, pyruvate, glyoxylate and alpha-ketobutyrate do not support the activity | Gluconobacter oxydans | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
the enzyme retains its full activity after heating for 10 min in 0.1 M potassium phosphate buffer, pH 6.8 | Gluconobacter oxydans |
55 | - |
10 min, in 0.1 M potassium phosphate buffer, pH 6.8, 70-80% loss of activity | Gluconobacter oxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Gluconobacter oxydans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Gluconobacter oxydans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Gluconobacter oxydans |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
DL-methionine DL-sulfoximine | competitive inhibitor with respect to alpha-keto-glutarate | Gluconobacter oxydans | |
0.07 | - |
NADP | competitive inhibition with NADPH | Gluconobacter oxydans | |
0.12 | - |
NADP | mixed-type inhibition with alpha-ketoglutarate | Gluconobacter oxydans | |
0.14 | - |
DL-methionine DL-sulfoximine | competitive inhibitor with respect to glutamine | Gluconobacter oxydans | |
0.3 | - |
NADP | uncompetitive inhibition with respect to glutamine | Gluconobacter oxydans | |
0.48 | - |
methionine | competitive inhibitor with respect to alpha-keto-glutarate | Gluconobacter oxydans | |
0.76 | - |
DL-methionine DL-sulfoximine | noncompetitive inhibitor with respect to NADPH | Gluconobacter oxydans | |
3 | - |
methionine | competitive inhibitor with respect to glutamine | Gluconobacter oxydans | |
5.5 | - |
methionine | noncompetitive inhibitor with respect to NADPH | Gluconobacter oxydans |