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Literature summary for 1.4.1.13 extracted from

  • Vanoni, M.A.; Edmondson, D.E.; Rescigno, M.; Zanetti, G.; Curti, B.
    Mechanistic studies on Azospirillum brasilense glutamate synthase (1991), Biochemistry, 30, 11478-11484.
    View publication on PubMed
Search for more literature for 1.4.1.13

Inhibitors

Inhibitors Comment Organism Structure
D-glutamate competitive inhibitor with respect to 2-oxoglutarate, noncompetitive with L-glutamine and uncompetitive with NADPH Azospirillum brasilense
L-methionine
-
 Azospirillum brasilense

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 thio-NADPH in the presence of 2.5 mM 2-oxo-glutarate and 5 mM L-glutamine, the apparent maximal velocity is 54% that obtained in the presence of NADPH Azospirillum brasilense
0.017
-
 acetylpyridine-NADPH in the presence of 2.5 mM 2-oxo-glutarate and 5 mM L-glutamine, the apparent maximal velocity is 3.7% that obtained in the presence of NADPH Azospirillum brasilense

Organism

Organism UniProt Comment Textmining
Azospirillum brasilense
-
-
-

Reaction

Reaction Comment Organism Reaction ID
2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+ mechanism Azospirillum brasilense
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 2-oxoglutarate + acetylpyridine-NADPH + H+
-
 Azospirillum brasilense L-glutamate + acetylpyridine-NADP+
-
 r
L-glutamine + 2-oxoglutarate + NADPH + H+
-
 Azospirillum brasilense L-glutamate + NADP+
-
 ir
L-glutamine + 2-oxoglutarate + thio-NADPH + H+
-
 Azospirillum brasilense L-glutamate + thio-NADP+
-
 r
additional information under conditions of physiological pH the enzyme exhibits a reversible half-reaction, but overall catalysis is essentially irreversible Azospirillum brasilense ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
additional information thio-NADPH and acetylpyridine-NADPH can be used as electron donors but are less efficient than NADPH Azospirillum brasilense
NADPH oxidizes NADPH stereospecifically at the 4S position Azospirillum brasilense

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.011
-
 D-glutamate good competitive inhibitor with respect to 2-oxoglutarate Azospirillum brasilense
0.044
-
 D-glutamate uncompetitive inhibitor with NADPH Azospirillum brasilense
0.056
-
 D-glutamate noncompetitive inhibitor with L-glutamine Azospirillum brasilense
1.05
-
 L-methionine competitive inhibitor with respect to L-glutamine Azospirillum brasilense