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Literature summary for 1.4.1.1 extracted from

  • Nakane, K.; Suye, S.; Ueno, T.; Ohno, Y.; Ishikawa, T.; Ogihara, T.; Ogata, N.
    Coimmobilization of malic enzyme and alanine dehydrogenase on organic-inorganic hybrid gel fibers and the production of L-alanine from malic acid using the fibers with coenzyme regeneration (2010), J. Appl. Polym. Sci., 116, 2901-2905.
No PubMed abstract available

Protein Variants

Protein Variants Comment Organism
additional information coimmobilization of malic enzyme and alanine dehydrogenase on organic-inorganic hybrid gel fibers of cellulose acetate and zirconium alkoxide by air-gap wet spinning, and the production of L-alanine from malic acid using the fibers with coenzyme regeneration, overview Geobacillus stearothermophilus

Inhibitors

Inhibitors Comment Organism Structure
MgCl2 inhibitory at a concentration above 2.13 mM Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-alanine + NAD+ + H2O Geobacillus stearothermophilus
-
pyruvate + NH3 + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation 55 U/mg Geobacillus stearothermophilus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine + NAD+ + H2O
-
Geobacillus stearothermophilus pyruvate + NH3 + NADH + H+
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Geobacillus stearothermophilus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Geobacillus stearothermophilus
NADH
-
Geobacillus stearothermophilus