Protein Variants | Comment | Organism |
---|---|---|
D198A | mutant enzyme shows higher efficiency with NADP+ as coenzyme than with NAD+ | Shewanella sp. |
D198G | mutant enzyme shows higher efficiency with NADP+ as coenzyme than with NAD+ | Shewanella sp. |
D198L | mutant enzyme shows higher efficiency with NADP+ as coenzyme than with NAD+ | Shewanella sp. |
D198R | acts also on NADP+ | Phormidium lapideum |
D198V | mutant enzyme shows higher efficiency with NADP+ as coenzyme than with NAD+ | Shewanella sp. |
R199I | specific for NAD+ as the wild-type enzyme | Phormidium lapideum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NAD+ | 30°C, mutant enzyme R199I | Shewanella sp. | |
0.035 | - |
NAD+ | 30°C, wild-type enzyme | Shewanella sp. | |
0.036 | - |
NAD+ | 45°C, wild-type enzyme | Phormidium lapideum | |
0.15 | - |
NAD+ | 45°C, mutant enzyme I198R | Phormidium lapideum | |
0.35 | - |
NADP+ | 30°C, mutant enzyme D198AI | Shewanella sp. | |
0.67 | - |
NADP+ | 30°C, mutant enzyme D198G | Shewanella sp. | |
1.1 | - |
NADP+ | 30°C, mutant enzyme D198V | Shewanella sp. | |
2.1 | - |
NADP+ | 30°C, mutant enzyme D198L | Shewanella sp. | |
2.8 | - |
NADP+ | 45°C, mutant enzyme I198R | Phormidium lapideum | |
3 | - |
NAD+ | 30°C, mutant enzyme D198G | Shewanella sp. | |
4 | - |
NADP+ | 30°C, wild-type enzyme | Shewanella sp. | |
5.2 | - |
NAD+ | 30°C, mutant enzyme D198V | Shewanella sp. | |
5.6 | - |
NAD+ | 30°C, mutant enzyme D198AI | Shewanella sp. | |
11.4 | - |
NAD+ | 30°C, mutant enzyme D198L | Shewanella sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phormidium lapideum | O52942 | Ac10 | - |
Phormidium lapideum Ac10 | O52942 | Ac10 | - |
Shewanella sp. | - |
Ac10 | - |
Shewanella sp. Ac10 | - |
Ac10 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala + H2O + NAD+ | - |
Shewanella sp. | pyruvate + NH3 + NADH | - |
? | |
L-Ala + H2O + NAD+ | - |
Phormidium lapideum | pyruvate + NH3 + NADH | - |
? | |
L-Ala + H2O + NAD+ | - |
Phormidium lapideum Ac10 | pyruvate + NH3 + NADH | - |
? | |
L-Ala + H2O + NAD+ | - |
Shewanella sp. Ac10 | pyruvate + NH3 + NADH | - |
? | |
L-Ala + H2O + NADP+ | wild-type enzyme and mutant enzyme R199I acts specifically on NAD+, mutant enzyme I198R is also active with NADP+ | Phormidium lapideum | pyruvate + NH3 + NADPH | - |
? | |
L-Ala + H2O + NADP+ | wild-type enzyme shows high activity with NAD+ and low activity with NADP+. The mutant enzymes D198G, D198A, D198V and D198L show higher efficiency with NADP+ than with NAD+ as coenzyme | Shewanella sp. | pyruvate + NH3 + NADPH | - |
? | |
L-Ala + H2O + NADP+ | wild-type enzyme and mutant enzyme R199I acts specifically on NAD+, mutant enzyme I198R is also active with NADP+ | Phormidium lapideum Ac10 | pyruvate + NH3 + NADPH | - |
? | |
L-Ala + H2O + NADP+ | wild-type enzyme shows high activity with NAD+ and low activity with NADP+. The mutant enzymes D198G, D198A, D198V and D198L show higher efficiency with NADP+ than with NAD+ as coenzyme | Shewanella sp. Ac10 | pyruvate + NH3 + NADPH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PlaAlaDH | - |
Phormidium lapideum |
SheAlaDH | - |
Shewanella sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
NADP+ | 45°C, mutant enzyme I198R | Phormidium lapideum | |
1.6 | - |
NADP+ | 30°C, wild-type enzyme | Shewanella sp. | |
1.9 | - |
NAD+ | 30°C, mutant enzyme D198L | Shewanella sp. | |
4.8 | - |
NADP+ | 30°C, mutant enzyme D198L | Shewanella sp. | |
9.5 | - |
NAD+ | 30°C, mutant enzyme R199I | Shewanella sp. | |
24.1 | - |
NADP+ | 30°C, mutant enzyme D198G | Shewanella sp. | |
34.7 | - |
NAD+ | 30°C, wild-type enzyme | Shewanella sp. | |
37.8 | - |
NADP+ | 30°C, mutant enzyme D198AI | Shewanella sp. | |
41.2 | - |
NAD+ | 30°C, mutant enzyme D198G | Shewanella sp. | |
41.2 | - |
NAD+ | 30°C, mutant enzyme D198V | Shewanella sp. | |
43.5 | - |
NAD+ | 30°C, mutant enzyme D198AI | Shewanella sp. | |
50.2 | - |
NADP+ | 30°C, mutant enzyme D198V | Shewanella sp. | |
95.4 | - |
NAD+ | 45°C, mutant enzyme I198R | Phormidium lapideum | |
95.5 | - |
NAD+ | 45°C, wild-type enzyme | Phormidium lapideum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | wild-type enzyme and mutant enzyme R199I acts specifically on NAD+, mutant enzyme I198R is also active with NADP+ | Phormidium lapideum | |
NAD+ | wild-type enzyme shows high activity with NAD+ and low activity with NADP+. The mutant enzymes D198G, D198A, D198V and D198L show higher efficiency with NADP+ than with NAD+ as coenzyme | Shewanella sp. | |
NADP+ | wild-type enzyme and mutant enzyme R199I acts specifically on NAD+, mutant enzyme I198R is also active with NADP+ | Phormidium lapideum | |
NADP+ | wild-type enzyme shows high activity with NAD+ and low activity with NADP+. The mutant enzymes D198G, D198A, D198V and D198L show higher efficiency with NADP+ than with NAD+ as coenzyme | Shewanella sp. |