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Literature summary for 1.4.1.1 extracted from

  • Galkin, A.; Kulakova, L.; Ashida, H.; Sawa, Y.; Esaki, N.
    Cold-adapted alanine dehydrogenases from two antarctic bacterial strains: gene cloning, protein characterization, and comparison with mesophilic and thermophilic counterparts (1999), Appl. Environ. Microbiol., 65, 4014-4020.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Shewanella sp.
expression in Escherichia coli Carnobacterium sp.

General Stability

General Stability Organism
enzyme is extremely unstable and easily lysed Carnobacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 NAD+
-
 Bacillus subtilis
0.2
-
 NAD+
-
 Carnobacterium sp.
0.21
-
 NAD+
-
 Vibrio proteolyticus
0.24
-
 NAD+
-
 Shewanella sp.
1.73
-
 L-Ala
-
 Bacillus subtilis
3.8
-
 L-Ala
-
 Carnobacterium sp.
7.6
-
 L-Ala
-
 Shewanella sp.
30
-
 L-Ala
-
 Vibrio proteolyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 6 * 43000, SDS-PAGE Shewanella sp.

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Carnobacterium sp.
-
-
-
Carnobacterium sp. St2
-
-
-
Geobacillus stearothermophilus
-
-
-
Shewanella sp.
-
-
-
Shewanella sp. Ac10
-
-
-
Vibrio proteolyticus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
40
-
-
 Shewanella sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala + H2O + NAD+
-
 Bacillus subtilis pyruvate + NH3 + NADH
-
 r
L-Ala + H2O + NAD+
-
 Geobacillus stearothermophilus pyruvate + NH3 + NADH
-
 r
L-Ala + H2O + NAD+
-
 Vibrio proteolyticus pyruvate + NH3 + NADH
-
 r
L-Ala + H2O + NAD+
-
 Shewanella sp. pyruvate + NH3 + NADH
-
 r
L-Ala + H2O + NAD+
-
 Carnobacterium sp. pyruvate + NH3 + NADH
-
 r
L-Ala + H2O + NAD+
-
 Carnobacterium sp. St2 pyruvate + NH3 + NADH
-
 r
L-Ala + H2O + NAD+
-
 Shewanella sp. Ac10 pyruvate + NH3 + NADH
-
 r

Subunits

Subunits Comment Organism
hexamer
-
 Bacillus subtilis
hexamer
-
 Geobacillus stearothermophilus
hexamer 6 * 43000, SDS-PAGE Shewanella sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 thermal instability may result from relatively low numbers of salt bridges in the enzymes Shewanella sp.
additional information
-
 thermal instability may result from relatively low numbers of salt bridges in the enzymes Carnobacterium sp.
41
-
 30 min, 50% loss of activity Carnobacterium sp.
59
-
 30 min, 50% loss of activity Shewanella sp.
63
-
-
 Bacillus subtilis
63
-
 30 min, 50% loss of activity Vibrio proteolyticus
64
-
 30 min, 50% loss of activity Bacillus subtilis
81
-
 30 min, 50% loss of activity Geobacillus stearothermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Bacillus subtilis
additional information
-
 additional information
-
 Shewanella sp.
additional information
-
 additional information
-
 Carnobacterium sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Bacillus subtilis
NAD+
-
 Geobacillus stearothermophilus
NAD+
-
 Vibrio proteolyticus
NAD+
-
 Shewanella sp.
NAD+
-
 Carnobacterium sp.
NADH
-
 Bacillus subtilis
NADH
-
 Geobacillus stearothermophilus
NADH
-
 Vibrio proteolyticus
NADH
-
 Shewanella sp.
NADH
-
 Carnobacterium sp.