General Stability | Organism |
---|---|
93% loss of activity after dialysis | Streptomyces clavuligerus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxobutanoate | weak | Streptomyces clavuligerus | |
3-hydroxypyruvate | competitive with respect to pyruvate | Streptomyces clavuligerus | |
Cu2+ | - |
Streptomyces clavuligerus | |
D-Ala | aminating reaction, competitive with respect to NADH, noncompetitive with respect to NH4+ and pyruvate | Streptomyces clavuligerus | |
Fe2+ | - |
Streptomyces clavuligerus | |
glyoxylate | weak | Streptomyces clavuligerus | |
Hg2+ | - |
Streptomyces clavuligerus | |
Hydroxypyruvate | - |
Streptomyces clavuligerus | |
L-Ala | reductive amination of pyruvate | Streptomyces clavuligerus | |
L-Ser | inhibition of aminating activity | Streptomyces clavuligerus | |
NADPH | inhibition of reductive amination | Streptomyces clavuligerus | |
NH4+ | inhibition of aminating activity | Streptomyces clavuligerus | |
Zn2+ | - |
Streptomyces clavuligerus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
NADH | - |
Streptomyces clavuligerus | |
0.5 | - |
pyruvate | - |
Streptomyces clavuligerus | |
1.1 | - |
pyruvate | - |
Streptomyces clavuligerus | |
9.1 | - |
L-Ala | - |
Streptomyces clavuligerus | |
20 | - |
NH4+ | - |
Streptomyces clavuligerus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
92000 | - |
sucrose density gradient centrifugation | Streptomyces clavuligerus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces clavuligerus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces clavuligerus |
Storage Stability | Organism |
---|---|
4°C, 75% saturated ammonium sulfate suspension, purified enzyme stable for at least 12 months | Streptomyces clavuligerus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutanoate + NH3 + NADH | no activity | Streptomyces clavuligerus | 2-aminobutanoate + H2O + NAD+ | - |
? | |
2-oxobutanoate + NH3 + NADH | 3.7% of the activity with pyruvate | Streptomyces clavuligerus | 2-aminobutanoate + H2O + NAD+ | - |
? | |
3-hydroxypyruvate + NH3 + NADH | 6.9% of the activity with pyruvate | Streptomyces clavuligerus | L-Ser + H2O + NAD+ | - |
? | |
glyoxylate + NH3 + NADH | 5.6% of the activity with pyruvate | Streptomyces clavuligerus | aminoacetate + NAD+ + H2O | - |
? | |
L-Ala + H2O + NAD+ | - |
Streptomyces clavuligerus | pyruvate + NH3 + NADH | - |
r | |
L-Ser + H2O + NAD+ | no activity | Streptomyces clavuligerus | 3-hydroxypyruvate + NH3 + NADH | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | 8.6 | pyruvate amination | Streptomyces clavuligerus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Streptomyces clavuligerus | |
NADH | - |
Streptomyces clavuligerus | |
NADP+ | 8% of the activity with NAD+ | Streptomyces clavuligerus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
L-Ala | with respect to NADH | Streptomyces clavuligerus | |
2 | - |
L-Ala | with respect to NH4+ | Streptomyces clavuligerus | |
3 | - |
L-Ala | with respect to pyruvate | Streptomyces clavuligerus |