General Stability | Organism |
---|---|
reversible deactivation by dilution | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | 0.1 mM, 57% inhibition | Bacillus subtilis | |
CaCl2 | 10 mM, 22% inhibition | Bacillus subtilis | |
CoCl2 | 0.1 mM, 53% inhibition | Bacillus subtilis | |
CuSO4 | 10 mM, 36% inhibition | Bacillus subtilis | |
HgCl2 | 0.003 mM, complete inhibition | Bacillus subtilis | |
MnCl2 | 10 mM, 12% inhibition | Bacillus subtilis | |
MnSO4 | 10 mM, 50% inhibition | Bacillus subtilis | |
PCMB | 0.3 mM, 52% inhibition | Bacillus subtilis | |
Zn2+ | 10 mM, 62% inhibition | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
NADH | - |
Bacillus subtilis | |
0.18 | - |
NAD+ | - |
Bacillus subtilis | |
0.53 | - |
pyruvate | - |
Bacillus subtilis | |
0.54 | - |
pyruvate | - |
Bacillus subtilis | |
1.73 | - |
L-Ala | - |
Bacillus subtilis | |
2.5 | - |
Hydroxypyruvate | - |
Bacillus subtilis | |
7 | - |
2-Oxohexanoate | - |
Bacillus subtilis | |
12 | - |
3-methyl-2-oxobutanoate | - |
Bacillus subtilis | |
16 | - |
glyoxalate | - |
Bacillus subtilis | |
23 | - |
2-oxobutanoate | - |
Bacillus subtilis | |
33 | - |
2-oxopentanoate | - |
Bacillus subtilis | |
38 | - |
NH4+ | - |
Bacillus subtilis | |
45 | - |
4-methyl-2-oxopentanoate | - |
Bacillus subtilis | |
57 | - |
L-Ser | - |
Bacillus subtilis | |
62 | - |
L-norvaline | - |
Bacillus subtilis | |
150 | - |
L-Val | - |
Bacillus subtilis | |
176 | - |
L-2-aminobutanoate | - |
Bacillus subtilis | |
250 | - |
L-Ile | - |
Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
220000 | 230000 | equilibrium sedimentation | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme assay | Bacillus subtilis |
Storage Stability | Organism |
---|---|
4°C, stable for many months | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-ketocaproate + NH3 + NADH | - |
Bacillus subtilis | 2-aminocaproate + H2O + NAD+ | - |
? | |
4-methyl-2-oxopentanoate + NH3 + NADH | - |
Bacillus subtilis | 2-amino-4-methylpentanoate + NAD+ + H2O | - |
? | |
glyoxalate + NH3 + NADH + H+ | - |
Bacillus subtilis | aminoacetate + H2O + NAD+ | - |
r | |
hydroxypyruvate + NH3 + NADH | - |
Bacillus subtilis | L-Ser + NAD+ + H2O | - |
? | |
L-2-aminobutanoate + H2O + NAD+ | 13.3% of the activity with L-Ala | Bacillus subtilis | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
L-Ala + H2O + NAD+ | - |
Bacillus subtilis | pyruvate + NH3 + NADH | - |
r | |
L-Ile + H2O + NAD+ | 5% of the activity with L-Ala | Bacillus subtilis | 2-oxo-3-methylpentanoate + NH3 + NADH | - |
? | |
L-norvaline + H2O + NAD+ | - |
Bacillus subtilis | 2-oxopentanoate + NH3 + NADH | - |
r | |
L-Ser + H2O + NAD+ | - |
Bacillus subtilis | 3-hydroxypyruvate + NH3 + NADH | - |
? | |
L-Val + H2O + NAD+ | 9% of the activity with L-Ala | Bacillus subtilis | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | amination: maximal turnover is about 650000 per min, deamination: maximal turnover is 54000 per min | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | 9 | amination | Bacillus subtilis |
10 | 10.5 | oxidative deamination | Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.2 | 10 | pH 7.2: about 10% of activity maximum, pH 10.0: about 30% of activity maximum, reductive amination | Bacillus subtilis |
8 | 11 | pH 8: about 35% of activity maximum, pH 11: about 75% of activity maximum, oxidative deamination | Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | - |
rapid and complete inactivation below | Bacillus subtilis |
5 | - |
stable above | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus subtilis | |
NADH | - |
Bacillus subtilis |