Cloned (Comment) | Organism |
---|---|
gene kstD1, heterologous expression of the His-tagged DELTA1-KSTD1 protein in Escherichia coli strain BL21(DE3) | Rhodococcus erythropolis |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapour diffusion method, mixing of 15 mg/ml protein in 25 mM bicine, pH 9.0, 100 mM NaCl, 10% v/v glycerol with 2% v/v PEG 400, 0.1 M, HEPES, pH 7.5, 2.0 M ammonium sulfate, 20°C, 5-7 days, method optimization, X-ray diffraction structure determination and analysis at 2.0 A resolution, multiwavelength anomalous dispersion analysis | Rhodococcus erythropolis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
x * 56000 | Rhodococcus erythropolis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-androstene-3,17-dione + FAD | Rhodococcus erythropolis | - |
1,4-androstadiene-3,17-dione + FADH2 | - |
? | |
4-androstene-3,17-dione + FAD | Rhodococcus erythropolis SQ1 | - |
1,4-androstadiene-3,17-dione + FADH2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | Q9RA02 | three 3-ketosteroid DELTA1-dehydrogenase isoenzymes, gene kstD1 encodes isozyme DELTA1-KSTD1 | - |
Rhodococcus erythropolis SQ1 | Q9RA02 | three 3-ketosteroid DELTA1-dehydrogenase isoenzymes, gene kstD1 encodes isozyme DELTA1-KSTD1 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged DELTA1-KSTD1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Rhodococcus erythropolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-androstene-3,17-dione + FAD | - |
Rhodococcus erythropolis | 1,4-androstadiene-3,17-dione + FADH2 | - |
? | |
4-androstene-3,17-dione + FAD | - |
Rhodococcus erythropolis SQ1 | 1,4-androstadiene-3,17-dione + FADH2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56000 | Rhodococcus erythropolis |
Synonyms | Comment | Organism |
---|---|---|
3-ketosteroid delta1-dehydrogenase | - |
Rhodococcus erythropolis |
DELTA1-KSTD1 | - |
Rhodococcus erythropolis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | below pH 6.0 no apparent Tm is observed, indicating that DELTA1-KSTD1 is destabilized at low pH. The higher the pH value, within the range of pH 6.0-9.0, the higher the apparent Tm of DELTA1-KSTD1. At pH 9.0 the apparent Tm of DELTA1-KSTD1 is about 7 K higher than at pH 7.0 | Rhodococcus erythropolis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | prosthetic group, flavoenzyme | Rhodococcus erythropolis |
General Information | Comment | Organism |
---|---|---|
physiological function | 3-ketosteroid DELTA1-dehydrogenase plays a crucial role in the early steps of steroid degradation by introducing a double bond between the C1 and C2 atoms of the A-ring of its 3-ketosteroid substrates | Rhodococcus erythropolis |