Cloned (Comment) | Organism |
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overexpression in Xanthophyllomyces dendrorhous. To achieve this, the plasmids pPR40F and pPR40R are introduced in Xanthophyllomyces dendrorhous CBS 6938. In the nontransformed strain, cyclization of lycopene, which directs the metabolic flux towards astaxanthin, is the dominating reaction. When the gene encoding phytoene desaturase is overexpressed, the five-step desaturation to 3,4-didehydrolycopene is intensified, resulting in an accumulation of torulene and 3-hydroxy-3'-4'-didehydro-beta-psi-caroten-4-one as subsequent products | Phaffia rhodozyma |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
15-cis-phytoene + 5 acceptor | Phaffia rhodozyma | a decisive reaction for the formation of monocyclic or bicyclic products is the desaturation sequence to lycopene and further on to 3,4-didehydrolycopene. In the nontransformed strain, cyclization of lycopene, which directs the metabolic flux towards astaxanthin, is the dominating reaction. When the gene encoding phytoene desaturase is overexpressed, the five-step desaturation to 3,4-didehydrolycopene is intensified, resulting in an accumulation of torulene and 3-hydroxy-3'-4'-didehydro-beta-psi-caroten-4-one as subsequent products | all-trans-3,4-didehydrolycopene + 5 reduced acceptor | - |
? | |
15-cis-phytoene + 5 acceptor | Phaffia rhodozyma CBS 6938 | a decisive reaction for the formation of monocyclic or bicyclic products is the desaturation sequence to lycopene and further on to 3,4-didehydrolycopene. In the nontransformed strain, cyclization of lycopene, which directs the metabolic flux towards astaxanthin, is the dominating reaction. When the gene encoding phytoene desaturase is overexpressed, the five-step desaturation to 3,4-didehydrolycopene is intensified, resulting in an accumulation of torulene and 3-hydroxy-3'-4'-didehydro-beta-psi-caroten-4-one as subsequent products | all-trans-3,4-didehydrolycopene + 5 reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
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Phaffia rhodozyma | - |
- |
- |
Phaffia rhodozyma CBS 6938 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
15-cis-phytoene + 5 acceptor | - |
Phaffia rhodozyma | all-trans-3,4-didehydrolycopene + 5 reduced acceptor | - |
? | |
15-cis-phytoene + 5 acceptor | a decisive reaction for the formation of monocyclic or bicyclic products is the desaturation sequence to lycopene and further on to 3,4-didehydrolycopene. In the nontransformed strain, cyclization of lycopene, which directs the metabolic flux towards astaxanthin, is the dominating reaction. When the gene encoding phytoene desaturase is overexpressed, the five-step desaturation to 3,4-didehydrolycopene is intensified, resulting in an accumulation of torulene and 3-hydroxy-3'-4'-didehydro-beta-psi-caroten-4-one as subsequent products | Phaffia rhodozyma | all-trans-3,4-didehydrolycopene + 5 reduced acceptor | - |
? | |
15-cis-phytoene + 5 acceptor | - |
Phaffia rhodozyma CBS 6938 | all-trans-3,4-didehydrolycopene + 5 reduced acceptor | - |
? | |
15-cis-phytoene + 5 acceptor | a decisive reaction for the formation of monocyclic or bicyclic products is the desaturation sequence to lycopene and further on to 3,4-didehydrolycopene. In the nontransformed strain, cyclization of lycopene, which directs the metabolic flux towards astaxanthin, is the dominating reaction. When the gene encoding phytoene desaturase is overexpressed, the five-step desaturation to 3,4-didehydrolycopene is intensified, resulting in an accumulation of torulene and 3-hydroxy-3'-4'-didehydro-beta-psi-caroten-4-one as subsequent products | Phaffia rhodozyma CBS 6938 | all-trans-3,4-didehydrolycopene + 5 reduced acceptor | - |
? | |
all-trans-lycopene + 1 acceptor | - |
Phaffia rhodozyma | all-trans-3,4-didehydrolycopene + 1 reduced acceptor | - |
? | |
all-trans-lycopene + 1 acceptor | - |
Phaffia rhodozyma CBS 6938 | all-trans-3,4-didehydrolycopene + 1 reduced acceptor | - |
? | |
all-trans-neurosporene + 2 acceptor | - |
Phaffia rhodozyma | all-trans-3,4-didehydrolycopene + 2 reduced acceptor | - |
? | |
all-trans-neurosporene + 2 acceptor | - |
Phaffia rhodozyma CBS 6938 | all-trans-3,4-didehydrolycopene + 2 reduced acceptor | - |
? | |
all-trans-phytofluene + 4 acceptor | - |
Phaffia rhodozyma | all-trans-3,4-didehydrolycopene + 4 reduced acceptor | - |
? | |
all-trans-phytofluene + 4 acceptor | - |
Phaffia rhodozyma CBS 6938 | all-trans-3,4-didehydrolycopene + 4 reduced acceptor | - |
? | |
all-trans-zeta-carotene + 3 acceptor | - |
Phaffia rhodozyma | all-trans-3,4-didehydrolycopene + 3 reduced acceptor | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CrtI | - |
Phaffia rhodozyma |