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Literature summary for 1.3.99.30 extracted from

  • Verdoes, J.C.; Sandmann, G.; Visser, H.; Diaz, M.; van Mossel, M.; van Ooyen, A.J.
    Metabolic engineering of the carotenoid biosynthetic pathway in the yeast Xanthophyllomyces dendrorhous (Phaffia rhodozyma) (2003), Appl. Environ. Microbiol., 69, 3728-3738.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Xanthophyllomyces dendrorhous. To achieve this, the plasmids pPR40F and pPR40R are introduced in Xanthophyllomyces dendrorhous CBS 6938. In the nontransformed strain, cyclization of lycopene, which directs the metabolic flux towards astaxanthin, is the dominating reaction. When the gene encoding phytoene desaturase is overexpressed, the five-step desaturation to 3,4-didehydrolycopene is intensified, resulting in an accumulation of torulene and 3-hydroxy-3'-4'-didehydro-beta-psi-caroten-4-one as subsequent products Phaffia rhodozyma

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
15-cis-phytoene + 5 acceptor Phaffia rhodozyma a decisive reaction for the formation of monocyclic or bicyclic products is the desaturation sequence to lycopene and further on to 3,4-didehydrolycopene. In the nontransformed strain, cyclization of lycopene, which directs the metabolic flux towards astaxanthin, is the dominating reaction. When the gene encoding phytoene desaturase is overexpressed, the five-step desaturation to 3,4-didehydrolycopene is intensified, resulting in an accumulation of torulene and 3-hydroxy-3'-4'-didehydro-beta-psi-caroten-4-one as subsequent products all-trans-3,4-didehydrolycopene + 5 reduced acceptor
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?
15-cis-phytoene + 5 acceptor Phaffia rhodozyma CBS 6938 a decisive reaction for the formation of monocyclic or bicyclic products is the desaturation sequence to lycopene and further on to 3,4-didehydrolycopene. In the nontransformed strain, cyclization of lycopene, which directs the metabolic flux towards astaxanthin, is the dominating reaction. When the gene encoding phytoene desaturase is overexpressed, the five-step desaturation to 3,4-didehydrolycopene is intensified, resulting in an accumulation of torulene and 3-hydroxy-3'-4'-didehydro-beta-psi-caroten-4-one as subsequent products all-trans-3,4-didehydrolycopene + 5 reduced acceptor
-
?

Organism

Organism UniProt Comment Textmining
Phaffia rhodozyma
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Phaffia rhodozyma CBS 6938
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
15-cis-phytoene + 5 acceptor
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Phaffia rhodozyma all-trans-3,4-didehydrolycopene + 5 reduced acceptor
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?
15-cis-phytoene + 5 acceptor a decisive reaction for the formation of monocyclic or bicyclic products is the desaturation sequence to lycopene and further on to 3,4-didehydrolycopene. In the nontransformed strain, cyclization of lycopene, which directs the metabolic flux towards astaxanthin, is the dominating reaction. When the gene encoding phytoene desaturase is overexpressed, the five-step desaturation to 3,4-didehydrolycopene is intensified, resulting in an accumulation of torulene and 3-hydroxy-3'-4'-didehydro-beta-psi-caroten-4-one as subsequent products Phaffia rhodozyma all-trans-3,4-didehydrolycopene + 5 reduced acceptor
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?
15-cis-phytoene + 5 acceptor
-
Phaffia rhodozyma CBS 6938 all-trans-3,4-didehydrolycopene + 5 reduced acceptor
-
?
15-cis-phytoene + 5 acceptor a decisive reaction for the formation of monocyclic or bicyclic products is the desaturation sequence to lycopene and further on to 3,4-didehydrolycopene. In the nontransformed strain, cyclization of lycopene, which directs the metabolic flux towards astaxanthin, is the dominating reaction. When the gene encoding phytoene desaturase is overexpressed, the five-step desaturation to 3,4-didehydrolycopene is intensified, resulting in an accumulation of torulene and 3-hydroxy-3'-4'-didehydro-beta-psi-caroten-4-one as subsequent products Phaffia rhodozyma CBS 6938 all-trans-3,4-didehydrolycopene + 5 reduced acceptor
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?
all-trans-lycopene + 1 acceptor
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Phaffia rhodozyma all-trans-3,4-didehydrolycopene + 1 reduced acceptor
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?
all-trans-lycopene + 1 acceptor
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Phaffia rhodozyma CBS 6938 all-trans-3,4-didehydrolycopene + 1 reduced acceptor
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?
all-trans-neurosporene + 2 acceptor
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Phaffia rhodozyma all-trans-3,4-didehydrolycopene + 2 reduced acceptor
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?
all-trans-neurosporene + 2 acceptor
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Phaffia rhodozyma CBS 6938 all-trans-3,4-didehydrolycopene + 2 reduced acceptor
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?
all-trans-phytofluene + 4 acceptor
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Phaffia rhodozyma all-trans-3,4-didehydrolycopene + 4 reduced acceptor
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?
all-trans-phytofluene + 4 acceptor
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Phaffia rhodozyma CBS 6938 all-trans-3,4-didehydrolycopene + 4 reduced acceptor
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?
all-trans-zeta-carotene + 3 acceptor
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Phaffia rhodozyma all-trans-3,4-didehydrolycopene + 3 reduced acceptor
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?

Synonyms

Synonyms Comment Organism
CrtI
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Phaffia rhodozyma