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Literature summary for 1.3.99.27 extracted from

  • Albrecht, M.; Ruther, A.; Sandmann, G.
    Purification and biochemical characterization of a hydroxyneurosporene desaturase involved in the biosynthetic pathway of the carotenoid spheroidene in Rhodobacter sphaeroides (1997), J. Bacteriol., 179, 7462-7467.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0134
-
1-hydroxyneurosporene pH 8.0, 30°C Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
x * 58000, SDS-PAGE Cereibacter sphaeroides

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1'-hydroxy-gamma-carotene + acceptor
-
Cereibacter sphaeroides 1'-hydroxytorulene + reduced acceptor
-
?
1-hydroxylycopene + acceptor
-
Cereibacter sphaeroides 1-hydroxy-3,4-didehydrolycopene + reduced acceptor
-
?
1-hydroxyneurosporene + acceptor when O2 is depleted less than 10% of the specific activity is retained. It is rather unlikely that molecular oxygen is the genuine cofactor for hydroxyneurosporene desaturation reactions in Rhodobacter cells when they reach their maximum carotenoid content under anaerobic conditions. Consequently, another, as-yet-unknown electron acceptor may exist in Rhodobacter species that replaces molecular oxygen during anaerobic growth Cereibacter sphaeroides demethylspheroidene + reduced acceptor
-
?
additional information 1'-hydroxy-3,4-didehydrolycopene and 1,1'-dihydroxylycopene are poor substrates. 1-Methoxyneurosporene is not converted by purified hydroxyneurosporene desaturase Cereibacter sphaeroides ?
-
?

Subunits

Subunits Comment Organism
? x * 58000, SDS-PAGE Cereibacter sphaeroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Cereibacter sphaeroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Cereibacter sphaeroides

Cofactor

Cofactor Comment Organism Structure
additional information NAD+, NADP+, and flavin adenine dinucleotide have no influence on enzymatic activity. When O2 is depleted less than 10% of the specific activity is retained. It is rather unlikely that molecular oxygen is the genuine cofactor for hydroxyneurosporene desaturation reactions in Rhodobacter cells when they reach their maximum carotenoid content under anaerobic conditions. Consequently, another, as-yet-unknown electron acceptor may exist in Rhodobacter species that replaces molecular oxygen during anaerobic growth Cereibacter sphaeroides

General Information

General Information Comment Organism
physiological function the enzyme is involved in the carotenoid biosynthetic pathway of Rhodobacter species Cereibacter sphaeroides