Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Acriflavine | - |
Pseudomonas sp. | |
CoCl2 | - |
Pseudomonas sp. | |
Cu2+ | - |
Pseudomonas sp. | |
KCN | 74% reactivation with S2- | Pseudomonas sp. | |
methanol | - |
Pseudomonas sp. | |
Mn2+ | - |
Pseudomonas sp. | |
NaAsO2 | - |
Pseudomonas sp. | |
NiCl2 | - |
Pseudomonas sp. | |
p-hydroxymercuribenzoate | - |
Pseudomonas sp. | |
Quinacrine | - |
Pseudomonas sp. | |
ZnSO4 | - |
Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
Quinaldate | with 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride as cosubstrate | Pseudomonas sp. | |
1.6 | - |
2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride | with quinaldate as cosubstrate | Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | contains 7.6 g-atom of iron per mol of enzyme and 7.5 g-atom of acid-labile sulfur per mol of enzyme | Pseudomonas sp. | |
Molybdenum | enzyme contains 1.6 g-atom per mol of enzyme | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE | Pseudomonas sp. |
32000 | - |
2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE | Pseudomonas sp. |
90000 | - |
2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE | Pseudomonas sp. |
300000 | - |
gel filtration | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
AK-2 | - |
Pseudomonas sp. AK-2 | - |
AK-2 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.82 | - |
- |
Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
quinaldate + 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride + H2O | - |
Pseudomonas sp. | kynurenate + reduced 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride | - |
r | |
quinaldate + 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride + H2O | - |
Pseudomonas sp. AK-2 | kynurenate + reduced 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride | - |
r | |
quinaldate + acceptor | acceptors: 1,4-benzoquinone, 1,2-naphthoquinone, nitro blue tetrazolium chloride, 2,6-dichlorophenol indophenol, thionine, menadione, 2,4,6-trinitrobenzo sulfonic acid | Pseudomonas sp. | kynurenate + reduced acceptor | i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate | ? | |
quinaldate + acceptor | i.e. quinoline 2-carboxylic acid | Pseudomonas sp. | kynurenate + reduced acceptor | i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate | ? | |
quinaldate + acceptor | acceptors: phenazine methosulfate, medola's blue, cytochrome c, 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride | Pseudomonas sp. | kynurenate + reduced acceptor | i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate | ? | |
quinaldate + acceptor | acceptors: 1,4-benzoquinone, 1,2-naphthoquinone, nitro blue tetrazolium chloride, 2,6-dichlorophenol indophenol, thionine, menadione, 2,4,6-trinitrobenzo sulfonic acid | Pseudomonas sp. AK-2 | kynurenate + reduced acceptor | i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate | ? | |
quinaldate + acceptor | i.e. quinoline 2-carboxylic acid | Pseudomonas sp. AK-2 | kynurenate + reduced acceptor | i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate | ? | |
quinaldate + acceptor | acceptors: phenazine methosulfate, medola's blue, cytochrome c, 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride | Pseudomonas sp. AK-2 | kynurenate + reduced acceptor | i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate | ? | |
quinoline 8-carboxylic acid + reduced acceptor | - |
Pseudomonas sp. | ? | - |
? | |
quinoline 8-carboxylic acid + reduced acceptor | - |
Pseudomonas sp. AK-2 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE | Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
above 60°C | Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
5 min, 32% loss of activity | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | enzyme contains 1.8 mol of FAD per mol of enzyme | Pseudomonas sp. | |
Pterin molybdenum cofactor | molybdenum is probably associated with molybdopterin cytosine dinucleotide as organic part of the cofactor, 2 mol of molybdopterin cytosine dinucleotide per mol of enzyme | Pseudomonas sp. |