Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-hydroxymercuribenzoate | - |
Rhodococcus sp. | |
CN- | - |
Pseudomonas putida | |
CN- | - |
Rhodococcus sp. | |
methanol | - |
Pseudomonas putida | |
methanol | - |
Rhodococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
Quinoline | - |
Rhodococcus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | contains 8 atoms of iron and 8 atoms of acid-labile sulfide | Pseudomonas putida | |
Fe | contains 8 atoms of iron and 8 atoms of acid-labile sulfide | Rhodococcus sp. | |
Mo | - |
Pseudomonas putida | |
Mo | the enzyme contains 1.3 mol of molybdenum per mol of enzyme | Rhodococcus sp. | |
Mo | enzyme contains 2 molecules of molybdopterin cytosine dinucleotide | Rhodococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
x * 18000 + x * 32000 + x * 82000, SDS-PAGE | Rhodococcus sp. |
32000 | - |
x * 18000 + x * 32000 + x * 82000, SDS-PAGE | Rhodococcus sp. |
82000 | - |
x * 18000 + x * 32000 + x * 82000, SDS-PAGE | Rhodococcus sp. |
270000 | - |
gel filtration | Rhodococcus sp. |
310000 | - |
native PAGE | Rhodococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida 86 | - |
- |
- |
Rhodococcus sp. | - |
- |
- |
Rhodococcus sp. B1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodococcus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.8 | - |
- |
Rhodococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3-methyl)-quinoline + acceptor | acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride | Pseudomonas putida | (3-methyl-)2-oxo-1,2-dihydroquinoline + reduced p-iodonitrotetrazolium violet | - |
? | |
(3-methyl)-quinoline + acceptor | 20% of the activity with quinoline | Pseudomonas putida | (3-methyl-)2-oxo-1,2-dihydroquinoline + reduced p-iodonitrotetrazolium violet | - |
? | |
4-methylquinoline + acceptor | acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride | Pseudomonas putida | ? | - |
? | |
4-methylquinoline + acceptor | 20% of the activity with quinoline | Pseudomonas putida | ? | - |
? | |
6-hydroxyquinoline + acceptor | acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride | Pseudomonas putida | ? | - |
? | |
6-hydroxyquinoline + acceptor | 56% of the activity with quinoline | Pseudomonas putida | ? | - |
? | |
6-hydroxyquinoline + acceptor | acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride | Pseudomonas putida 86 | ? | - |
? | |
6-hydroxyquinoline + acceptor | 56% of the activity with quinoline | Pseudomonas putida 86 | ? | - |
? | |
7-hydroxyquinoline + acceptor | acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride | Pseudomonas putida | ? | - |
? | |
7-hydroxyquinoline + acceptor | 27% of the activity with quinoline | Pseudomonas putida | ? | - |
? | |
7-hydroxyquinoline + acceptor | acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride | Pseudomonas putida 86 | ? | - |
? | |
7-hydroxyquinoline + acceptor | 27% of the activity with quinoline | Pseudomonas putida 86 | ? | - |
? | |
8-chloroquinoline + acceptor | acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride | Pseudomonas putida | ? | - |
? | |
8-chloroquinoline + acceptor | 34% of the activity with quinoline | Pseudomonas putida | ? | - |
? | |
8-hydroxyquinoline + acceptor | acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride | Pseudomonas putida | ? | - |
? | |
8-hydroxyquinoline + acceptor | 25% of the activity with quinoline | Pseudomonas putida | ? | - |
? | |
8-methylquinoline + acceptor | acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride | Pseudomonas putida | ? | - |
? | |
8-methylquinoline + acceptor | acceptor: p-iodonitrotetrazolium violet | Pseudomonas putida | ? | - |
? | |
8-methylquinoline + acceptor | 22% of the activity with quinoline | Pseudomonas putida | ? | - |
? | |
quinoline + acceptor | acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride | Pseudomonas putida | 2-oxo-1,2-dihydroquinoline + reduced acceptor | - |
? | |
quinoline + acceptor | acceptor: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride | Pseudomonas putida 86 | 2-oxo-1,2-dihydroquinoline + reduced acceptor | - |
? | |
quinoline + acceptor + H2O | - |
Rhodococcus sp. | quinolin-2(1H)-one + reduced acceptor | - |
r | |
quinoline + acceptor + H2O | - |
Rhodococcus sp. B1 | quinolin-2(1H)-one + reduced acceptor | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | - |
Pseudomonas putida |
? | x * 18000 + x * 32000 + x * 82000, SDS-PAGE | Rhodococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Rhodococcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | enzyme contains 1.8 mol FAD per mol of enzyme | Rhodococcus sp. | |
FAD | enzyme contains 2 moleculs of FAD | Pseudomonas putida | |
FAD | enzyme contains 2 moleculs of FAD | Rhodococcus sp. | |
molybdopterin | - |
Pseudomonas putida | |
molybdopterin | enzyme contains 2 molecules of molybdopterin cytosine dinucleotide | Rhodococcus sp. |