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Literature summary for 1.3.98.1 extracted from

  • Zameitat, E.; Pierik, A.J.; Zocher, K.; Loeffler, M.
    Dihydroorotate dehydrogenase from Saccharomyces cerevisiae: spectroscopic investigations with the recombinant enzyme throw light on catalytic properties and metabolism of fumarate analogues (2007), FEMS Yeast Res., 7, 897-904.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21-RP Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
L-orotate
-
Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
L-dihydroorotate in 50mM Tris/HCl, 150 mM KCl, 0.1% (v/v) Triton X-100, pH 8, at 30°C Saccharomyces cerevisiae
0.115
-
2,6-dichloroindophenol in 50mM Tris/HCl, 150 mM KCl, 0.1% (v/v) Triton X-100, pH 8, at 30°C Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33900
-
2 * 33900, theoretical molecular mass Saccharomyces cerevisiae
67000
-
SDS-PAGE Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA agarose chromatography, Q-Sepharose column chromatography, and Superdex 200 gel filtration Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.06
-
using crotonate as electron acceptor Saccharomyces cerevisiae
0.94
-
using mesaconate as electron acceptor Saccharomyces cerevisiae
1.81
-
using O2 as electron acceptor Saccharomyces cerevisiae
5.1
-
using fumarate as electron acceptor Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-dihydroorotate + 2,6-dichlorophenolindophenol
-
Saccharomyces cerevisiae orotate + reduced 2,6-dichlorophenolindophenol
-
?
L-dihydroorotate + crotonate
-
Saccharomyces cerevisiae orotate + butanoate
-
?
L-dihydroorotate + fumarate fumarate is the most effective electron acceptor Saccharomyces cerevisiae orotate + succinate
-
?
L-dihydroorotate + mesaconate
-
Saccharomyces cerevisiae orotate + 2-methylbutanedioate
-
?
L-dihydroorotate + O2
-
Saccharomyces cerevisiae orotate + H2O2
-
?
additional information maleate, glutaconate, cis-aconitate, trans-aconitate, and cinnamate do not function as electron acceptor Saccharomyces cerevisiae ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 33900, theoretical molecular mass Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
DHODH
-
Saccharomyces cerevisiae
DHODH-1A
-
Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
flavin contains 0.5 mol flavin per mol protein Saccharomyces cerevisiae

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0077
-
in 50mM Tris/HCl, 150 mM KCl, 0.1% (v/v) Triton X-100, pH 8, at 30°C Saccharomyces cerevisiae L-orotate