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Literature summary for 1.3.98.1 extracted from

  • Feliciano, P.R.; Cordeiro, A.T.; Costa-Filho, A.J.; Nonato, M.C.
    Cloning, expression, purification, and characterization of Leishmania major dihydroorotate dehydrogenase (2006), Protein Expr. Purif., 48, 98-103.
    View publication on PubMed

Application

Application Comment Organism
additional information the gene LMjF16.0530 possesses a dihydroorotate oxidase activity using fumarate as oxidizing agent Leishmania major

Cloned(Commentary)

Cloned (Comment) Organism
into vector pET-28a and overexpressed in Escherichia coli strain BL21(DE3) as a histidine-tag fusion protein Leishmania major

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0192
-
dihydroorotate in the presence of 0.125 mM fumarate Leishmania major
0.0332
-
dihydroorotate in the presence of 0.25 mM fumarate Leishmania major
0.0472
-
dihydroorotate in the presence of 0.5 mM fumarate Leishmania major
0.0579
-
dihydroorotate in the presence of 1 mM fumarate Leishmania major
0.0718
-
fumarate in the presence of 0.02 mM dihydroorotate Leishmania major
0.0975
-
fumarate in the presence of 0.03 mM dihydroorotate Leishmania major
0.1282
-
fumarate in the presence of 0.04 mM dihydroorotate Leishmania major
0.4135
-
fumarate in the presence of 0.05 mM dihydroorotate Leishmania major

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31600
-
2 * 31600 Leishmania major
63200
-
gel filtration Leishmania major

Organism

Organism UniProt Comment Textmining
Leishmania major
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity by a single affinity chromatography step Leishmania major

Source Tissue

Source Tissue Comment Organism Textmining
promastigote
-
Leishmania major
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.12
-
-
Leishmania major

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydroorotate + fumarate
-
Leishmania major orotate + reduced fumarate
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 31600 Leishmania major

Synonyms

Synonyms Comment Organism
DHODH
-
Leishmania major