Application | Comment | Organism |
---|---|---|
additional information | the gene LMjF16.0530 possesses a dihydroorotate oxidase activity using fumarate as oxidizing agent | Leishmania major |
Cloned (Comment) | Organism |
---|---|
into vector pET-28a and overexpressed in Escherichia coli strain BL21(DE3) as a histidine-tag fusion protein | Leishmania major |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0192 | - |
dihydroorotate | in the presence of 0.125 mM fumarate | Leishmania major | |
0.0332 | - |
dihydroorotate | in the presence of 0.25 mM fumarate | Leishmania major | |
0.0472 | - |
dihydroorotate | in the presence of 0.5 mM fumarate | Leishmania major | |
0.0579 | - |
dihydroorotate | in the presence of 1 mM fumarate | Leishmania major | |
0.0718 | - |
fumarate | in the presence of 0.02 mM dihydroorotate | Leishmania major | |
0.0975 | - |
fumarate | in the presence of 0.03 mM dihydroorotate | Leishmania major | |
0.1282 | - |
fumarate | in the presence of 0.04 mM dihydroorotate | Leishmania major | |
0.4135 | - |
fumarate | in the presence of 0.05 mM dihydroorotate | Leishmania major |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31600 | - |
2 * 31600 | Leishmania major |
63200 | - |
gel filtration | Leishmania major |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania major | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity by a single affinity chromatography step | Leishmania major |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
promastigote | - |
Leishmania major | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.12 | - |
- |
Leishmania major |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroorotate + fumarate | - |
Leishmania major | orotate + reduced fumarate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 31600 | Leishmania major |
Synonyms | Comment | Organism |
---|---|---|
DHODH | - |
Leishmania major |