Activating Compound | Comment | Organism | Structure |
---|---|---|---|
IPTG | induces expression | Trypanosoma cruzi |
Cloned (Comment) | Organism |
---|---|
ligated into the pZErO-2 vector and transformation of the Escherichia coli strain TOP 10, inserted into the pET3a expression vector and transformation of Escherichia coli BL21 | Trypanosoma cruzi |
Crystallization (Comment) | Organism |
---|---|
crystals of the TcDHODorotate complex are grown at 4°C by the sitting-drop vapour-diffusion technique using polyethylene glycol 3350 as a precipitant. The crystals diffract to better than 1.8 A ° resolution using synchrotron radiation (lambda = 0.900 A). X-ray diffraction data are collected at -173°C and processed to 1.9 A ° resolution with 98.2% completeness. The TcDHOD crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 67.87, b = 71.89, c = 123.27 A | Trypanosoma cruzi |
DHODorotate complex by the sitting-drop vapour-diffusion technique using polyethylene glycol 3350 as a precipitant, to better than 1.8 A resolution, crystals belong to the orthorhombic space group P212121, with unit-cell parameters a = 67.87, b = 71.89, c = 123.27 A | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
- |
- |
Trypanosoma cruzi | Q4D3W2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant | Trypanosoma cruzi |
to homogeneity by ion-exchange, hydrophobic interaction and gel filtration | Trypanosoma cruzi |
Synonyms | Comment | Organism |
---|---|---|
DHOD2 | - |
Trypanosoma cruzi |
TcDHOD | - |
Trypanosoma cruzi |