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Literature summary for 1.3.98.1 extracted from

  • Norager, S.; Arent, S.; Bjornberg, O.; Ottosen, M.; Lo Leggio, L.; Jensen, K.F.; Larsen, S.
    Lactococcus lactis dihydroorotate dehydrogenase A mutants reveal important facets of the enzymatic function (2003), J. Biol. Chem., 278, 28812-28822.
    View publication on PubMed
Search for more literature for 1.3.98.1

Crystallization (Commentary)

Crystallization (Comment) Organism
wild-type enzyme and mutants N67A, K213E, P56A, R57A, K136E Lactococcus lactis

Protein Variants

Protein Variants Comment Organism
K136E little changes in activity Lactococcus lactis
K213E decrease in activity against dichlorophenolindophenol Lactococcus lactis
N127A reduced activity Lactococcus lactis
N193A drastically reduced activiy Lactococcus lactis
N67A drastically reduced activiy Lactococcus lactis
P131A decrease in activtiy against fumarate Lactococcus lactis
P56A drastically reduced activiy Lactococcus lactis
R50E little changes in activity Lactococcus lactis
R57A increase in activity Lactococcus lactis
S129A decrease in activtiy against fumarate Lactococcus lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
 class 1A enzyme
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.09
-
 pH 8.0, 25°C, mutant N67A, substrate O2 Lactococcus lactis
0.11
-
 pH 8.0, 25°C, mutant N67A, substrate dichlorophenolindophenol Lactococcus lactis
0.14
-
 pH 8.0, 25°C, mutant N193A, substrate fumarate Lactococcus lactis
0.15
-
 pH 8.0, 25°C, mutant N193A, substrate dichlorophenolindophenol Lactococcus lactis
0.15
-
 pH 8.0, 25°C, mutant P56A, substrate O2 Lactococcus lactis
0.17
-
 pH 8.0, 25°C, mutant N193A, substrate O2 Lactococcus lactis
0.3
-
 pH 8.0, 25°C, mutant N67A, substrate fumarate Lactococcus lactis
0.47
-
 pH 8.0, 25°C, mutant P56A, substrate dichlorophenolindophenol Lactococcus lactis
0.48
-
 pH 8.0, 25°C, mutant P56A, substrate fumarate Lactococcus lactis
0.75
-
 pH 8.0, 25°C, mutant N127A, substrate dichlorophenolindophenol Lactococcus lactis
1
-
 pH 8.0, 25°C, mutant N127A, substrate O2 Lactococcus lactis
1.2
-
 pH 8.0, 25°C, mutant N127A, substrate fumarate Lactococcus lactis
1.6
-
 pH 8.0, 25°C, mutant K136E, substrate O2 Lactococcus lactis
1.8
-
 pH 8.0, 25°C, mutant S129A, substrate O2 Lactococcus lactis
1.9
-
 pH 8.0, 25°C, mutant K213E, substrate O2 Lactococcus lactis
2
-
 pH 8.0, 25°C, wild-type, substrate O2 Lactococcus lactis
2.3
-
 pH 8.0, 25°C, mutant P131A, substrate O2 Lactococcus lactis
2.4
-
 pH 8.0, 25°C, mutant K213E, substrate dichlorophenolindophenol Lactococcus lactis
2.8
-
 pH 8.0, 25°C, mutant S129A, substrate fumarate Lactococcus lactis
3.3
-
 pH 8.0, 25°C, mutant R50E, substrate O2 Lactococcus lactis
3.3
-
 pH 8.0, 25°C, mutant R57A, substrate O2 Lactococcus lactis
3.7
-
 pH 8.0, 25°C, mutant P131A, substrate fumarate Lactococcus lactis
4
-
 pH 8.0, 25°C, mutant P131A, substrate dichlorophenolindophenol Lactococcus lactis
7.2
-
 pH 8.0, 25°C, mutant K213E, substrate fumarate Lactococcus lactis
7.2
-
 pH 8.0, 25°C, mutant R50E, substrate dichlorophenolindophenol Lactococcus lactis
11.7
-
 pH 8.0, 25°C, mutant S129A, substrate dichlorophenolindophenol Lactococcus lactis
13
-
 pH 8.0, 25°C, mutant K136E, substrate dichlorophenolindophenol Lactococcus lactis
13.6
-
 pH 8.0, 25°C, wild-type, substrate dichlorophenolindophenol Lactococcus lactis
15.8
-
 pH 8.0, 25°C, wild-type, substrate fumarate Lactococcus lactis
18.1
-
 pH 8.0, 25°C, mutant K136E, substrate fumarate Lactococcus lactis
19
-
 pH 8.0, 25°C, mutant R50E, substrate fumarate Lactococcus lactis
27
-
 pH 8.0, 25°C, mutant R57A, substrate fumarate Lactococcus lactis
65.7
-
 pH 8.0, 25°C, mutant R57A, substrate dichlorophenolindophenol Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydroorotate + 2,6-dichlorophenolindophenol
-
 Lactococcus lactis orotate + reduced 2,6-dichlorophenolindophenol
-
 ?
dihydroorotate + fumarate
-
 Lactococcus lactis orotate + ?
-
 ?
dihydroorotate + O2
-
 Lactococcus lactis orotate + H2O2
-
 ?