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Literature summary for 1.3.98.1 extracted from

  • Nielsen, F.S.; Rowland, P.; Larsen, S.; Jensen, K.F.
    Purification and characterization of dihydroorotate dehydrogenase A from Lactococcus lactis, crystallization and preliminary X-ray diffraction studies of the enzyme (1996), Protein Sci., 5, 852-856.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.98.1

Cloned(Commentary)

Cloned (Comment) Organism
transformation in Escherichia coli for detection of pyrimidine prototrophic colonies with a resistance of ampicillin Lactococcus lactis

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Lactococcus lactis
hanging drop vapor diffusion technique, 30% polyethylene glycol, 0.2 M sodium acetate, 0.1 M Tris-HCl, pH 8.5, monoclinic crystals, space group P21 Lactococcus lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 2 * 34000, SDS-PAGE Lactococcus lactis
66000
-
 gel filtration Lactococcus lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-dihydroorotate + acceptor Lactococcus lactis fourth step in UMP-biosynthesis orotate + reduced acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
-
-
Lactococcus lactis
-
 only enzyme A
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lactococcus lactis
streptomycin precipitation, ion-exchange, hydroxylapatite Lactococcus lactis

Reaction

Reaction Comment Organism Reaction ID
(S)-dihydroorotate + fumarate = orotate + succinate ping-pong mechanism Lactococcus lactis
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17
-
-
 Lactococcus lactis

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol, several months, without loss of activity Lactococcus lactis
-20°C, 50% glycerol, stable for several months Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-dihydroorotate + acceptor 2,6-dichlorophenolindophenol, potassium hexacyanoferrate(III) and molecular oxygen used as electron acceptors Lactococcus lactis orotate + reduced acceptor
-
 ?
(S)-dihydroorotate + acceptor fourth step in UMP-biosynthesis Lactococcus lactis orotate + reduced acceptor
-
 ?
dihydroorotate + acceptor acceptor: 2,6-dichlorophenolindophenol Lactococcus lactis orotate + reduced acceptor
-
 ?
dihydroorotate + acceptor not acceptors: NAD+, NADP+, coenzymes Q6, Q10, K2 Lactococcus lactis orotate + reduced acceptor
-
 ?
dihydroorotate + acceptor no substrate: dihydrouracil Lactococcus lactis orotate + reduced acceptor
-
 ?
dihydroorotate + acceptor acceptors: potassium hexacyanoferrate(III), to a lower extent molecular oxygen Lactococcus lactis orotate + reduced acceptor
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 34000, SDS-PAGE Lactococcus lactis
dimer 2 * 34000-36000, SDS-PAGE Lactococcus lactis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Lactococcus lactis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 20 min, loss of half of the activity Lactococcus lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Lactococcus lactis
7.5 9
-
 Lactococcus lactis

pH Range

pH Minimum pH Maximum Comment Organism
6 10.5 assay in this range Lactococcus lactis
7.5 9
-
 Lactococcus lactis

Cofactor

Cofactor Comment Organism Structure
FMN one molecule per subunit Lactococcus lactis