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Literature summary for 1.3.98.1 extracted from

  • Bjornberg, O.; Jordan, D.B.; Palfey, B.A.; Jensen, K.F.
    Dihydrooxonate is a substrate of dihydroorotate dehydrogenase (DHOD) providing evidence for involvement of cysteine and serine residues in base catalysis (2001), Arch. Biochem. Biophys., 391, 286-294.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
C130A low activity with dihydroorotate, increasing activity above pH 8.0 with dihydrooxonate Lactococcus lactis
S175A
-
Lactococcus lactis
S175A low activity with dihydroorotate, increasing activity above pH 8.0 with dihydrooxonate Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
family 2 enzyme
-
Lactococcus lactis
-
family 1 enzymes A and B
-

Reaction

Reaction Comment Organism Reaction ID
(S)-dihydroorotate + fumarate = orotate + succinate cysteine and serine are active site base residues. One-site ping-pong mechanism with fumarate as electron acceptor Escherichia coli
(S)-dihydroorotate + fumarate = orotate + succinate cysteine and serine are active site base residues. One-site ping-pong mechanism with fumarate as electron acceptor Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-dihydroorotate + acceptor fumarate and ferricyanide tested as electron acceptors Escherichia coli orotate + reduced acceptor
-
?
(S)-dihydroorotate + acceptor fumarate and ferricyanide tested as electron acceptors Lactococcus lactis orotate + reduced acceptor
-
?
dihydrooxonate + acceptor fumarate and ferricyanide tested as electron acceptors Escherichia coli oxonate + reduced acceptor
-
?
dihydrooxonate + acceptor fumarate and ferricyanide tested as electron acceptors Lactococcus lactis oxonate + reduced acceptor
-
?

Synonyms

Synonyms Comment Organism
DHOD
-
Escherichia coli
DHOD
-
Lactococcus lactis