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Literature summary for 1.3.8.9 extracted from

  • Li, Z.; Zhai, Y.; Fang, J.; Zhou, Q.; Geng, Y.; Sun, F.
    Purification, crystallization and preliminary crystallographic analysis of very-long-chain acyl-CoA dehydrogenase from Caenorhabditis elegans (2010), Acta Crystallogr. Sect. F, 66, 426-430.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.8.9

Cloned(Commentary)

Cloned (Comment) Organism
cloning from cDNA library, His8-tagged VLCAD expression in Escherichia coli strain BL21 (DE3) Caenorhabditis elegans

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant cVLCAD, hanging-drop vapour-diffusion method, 0.002 ml of 12 mg/ml protein in 20 mM Tris-HCl pH 8.0, 150 mM NaCl are mixed with 0.002 ml of reservoir solution containing 100 mM Tris-HCl, pH 8.0, 150 mM NaCl, 200 mM magnesium formate and 13% PEG 3350, 16°C, 3 days, X-ray diffraction structure determination and analysis at 1.8-2.6 A resolution, molecular replacement Caenorhabditis elegans

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Caenorhabditis elegans 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
180000
-
 about, size-exclusion chromatography coupled with in-line static light-scattering, refractive-index and ultraviolet measurements Caenorhabditis elegans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Caenorhabditis elegans acyl-CoA dehydrogenases, ACADs, constitute a family of FAD-dependent flavoproteins that catalyze the alpha,beta-dehydrogenation of thioester substrates ?
-
 ?

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His8-tagged VLCAD from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography, anion exchange chromatography, and gel filtration Caenorhabditis elegans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information acyl-CoA dehydrogenases, ACADs, constitute a family of FAD-dependent flavoproteins that catalyze the alpha,beta-dehydrogenation of thioester substrates Caenorhabditis elegans ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 x 45000, about, size-exclusion chromatography coupled with in-line static light-scattering, refractive-index and ultraviolet measurements Caenorhabditis elegans

Synonyms

Synonyms Comment Organism
acyl-CoA:(acceptor) 2,3-oxidoreductase
-
 Caenorhabditis elegans
very-long-chain acyl-CoA dehydrogenase
-
 Caenorhabditis elegans
VLCAD
-
 Caenorhabditis elegans

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Caenorhabditis elegans