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Literature summary for 1.3.8.8 extracted from

  • Tani, A.; Ishige, T.; Sakai, Y.; Kato, N.
    Two acyl-CoA dehydrogenases of Acinetobacter sp. strain M-1 that uses very long-chain n-alkanes (2002), J. Biosci. Bioeng., 94, 326-329.
    View publication on PubMed
Search for more literature for 1.3.8.8

Cloned(Commentary)

Cloned (Comment) Organism
gene acdB, DNA and amino acid sequence determination and analysis, genes acdA and acdB are arranged in a tandem, expression of gene acdB in Escherichia coli strain JM109 Acinetobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, complete inhibition of the recombinant enzyme Acinetobacter sp.
Cu2+ 1 mM, complete inhibition of the recombinant enzyme Acinetobacter sp.
Hg2+ 1 mM, 61% inhibition of the recombinant enzyme Acinetobacter sp.
Mn2+ 1 mM, 72% inhibition of the recombinant enzyme Acinetobacter sp.
Pb2+ 1 mM, 23% inhibition of the recombinant enzyme Acinetobacter sp.
Zn2+ 1 mM, 19% inhibition of the recombinant enzyme Acinetobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.068
-
 lauroyl-CoA pH 8.5, recombinant enzyme Acinetobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
 2 * 64833, amino acid sequence determination, 2 * 64000, recombinant enzyme, SDS-PAGE Acinetobacter sp.
64833
-
 2 * 64833, amino acid sequence determination, 2 * 64000, recombinant enzyme, SDS-PAGE Acinetobacter sp.
135000
-
 recombinant enzyme, gel filtration Acinetobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lauroyl-CoA + acceptor Acinetobacter sp. enzyme shows preference for long-chain fatty acids 2-dodecenoyl-CoA + reduced acceptor
-
 ?
lauroyl-CoA + acceptor Acinetobacter sp. M-1 enzyme shows preference for long-chain fatty acids 2-dodecenoyl-CoA + reduced acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Acinetobacter sp. Q8L0X1 gene acdB
-
Acinetobacter sp. M-1 Q8L0X1 gene acdB
-

Purification (Commentary)

Purification (Comment) Organism
recombinant acdB-encoded enzyme from Escherichia coli Acinetobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lauroyl-CoA + acceptor enzyme shows preference for long-chain fatty acids Acinetobacter sp. 2-dodecenoyl-CoA + reduced acceptor
-
 ?
lauroyl-CoA + acceptor enzyme shows preference for long-chain fatty acids Acinetobacter sp. M-1 2-dodecenoyl-CoA + reduced acceptor
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 64833, amino acid sequence determination, 2 * 64000, recombinant enzyme, SDS-PAGE Acinetobacter sp.

Synonyms

Synonyms Comment Organism
AcdB
-
 Acinetobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 purified recombinant enzyme, 30 min, 80% remaining activity Acinetobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Acinetobacter sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 10 recombinant enzyme Acinetobacter sp.

Cofactor

Cofactor Comment Organism Structure
FAD 1 mol per mol of subunit, participates directly in the catalytic reaction Acinetobacter sp.