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Literature summary for 1.3.8.7 extracted from

  • Zeng, J.; Liu, Y.; Wu, L.; Li, D.
    Mutation of Tyr375 to Lys375 allows medium-chain acyl-CoA dehydrogenase to acquire acyl-CoA oxidase activity (2007), Biochim. Biophys. Acta, 1774, 1628-1634.
    View publication on PubMed
Search for more literature for 1.3.8.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
Y375A very low acyl-CoA dehydrogenase activity Rattus norvegicus
Y375E inactive Rattus norvegicus
Y375F very low acyl-CoA dehydrogenase activity Rattus norvegicus
Y375K very low acyl-CoA dehydrogenase activity, mutant exhibits acyl-CoA oxidase activity Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00084
-
 octanoyl-CoA mutant enzyme Y375K, in 50 mM potassium phosphate buffer, pH 7.6 Rattus norvegicus
0.0023
-
 octanoyl-CoA wild type enzyme, in 50 mM potassium phosphate buffer, pH 7.6 Rattus norvegicus
0.00242
-
 octanoyl-CoA mutant enzyme Y375F, in 50 mM potassium phosphate buffer, pH 7.6 Rattus norvegicus
0.00684
-
 octanoyl-CoA mutant enzyme Y375A, in 50 mM potassium phosphate buffer, pH 7.6 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap chelating nickel affinity chromatography Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Storage Stability

Storage Stability Organism
-80°C, 6 months, remains stable Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + acceptor
-
 Rattus norvegicus 2,3-dehydroacyl-CoA + reduced acceptor
-
 ?
hexanoyl-CoA + 2,6-dichloroindophenol
-
 Rattus norvegicus 2-hexenoyl-CoA + reduced 2,6-dichloroindophenol
-
 ?
octanoyl-CoA + 2,6-dichloroindophenol optimal substrate for wild type enzyme Rattus norvegicus 2-octenoyl-CoA + reduced 2,6-dichloroindophenol
-
 ?

Synonyms

Synonyms Comment Organism
MCAD
-
 Rattus norvegicus
medium chain acyl-CoA dehydrogenase
-
 Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.038
-
 octanoyl-CoA mutant enzyme Y375K, in 50 mM potassium phosphate buffer, pH 7.6 Rattus norvegicus
0.22
-
 octanoyl-CoA mutant enzyme Y375A, in 50 mM potassium phosphate buffer, pH 7.6 Rattus norvegicus
0.37
-
 octanoyl-CoA mutant enzyme Y375F, in 50 mM potassium phosphate buffer, pH 7.6 Rattus norvegicus
3.3
-
 octanoyl-CoA wild type enzyme, in 50 mM potassium phosphate buffer, pH 7.6 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Rattus norvegicus