Crystallization (Comment) | Organism |
---|---|
dialysis against distilled water at 4°C | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyldicarbonate | rapid inactivation | Sus scrofa | |
iodoacetic acid | S-carboxymethylated enzyme binds octanoyl-CoA but can no longer be reduced by it | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
4 * 42000, SDS-PAGE | Sus scrofa |
160000 | - |
gel filtration | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4 | - |
- |
Sus scrofa |
Storage Stability | Organism |
---|---|
-20°C, 100 mM phosphate buffer, pH 7.6 | Sus scrofa |
4°C, ammonium sulfate suspension | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + acceptor | electron acceptor: phenazine methosulfate | Sus scrofa | 2,3-dehydroacyl-CoA + reduced acceptor | - |
? | |
acyl-CoA + acceptor | intermediary electron acceptor: 2,6-dichloroindophenol | Sus scrofa | 2,3-dehydroacyl-CoA + reduced acceptor | - |
? | |
acyl-CoA + acceptor | final electron acceptor: 8-dimethylamino-2,3-benzophenoxazinium chloride, trivial name meldola's blue | Sus scrofa | 2,3-dehydroacyl-CoA + reduced acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 42000, SDS-PAGE | Sus scrofa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.25 | - |
octanoyl-CoA | phenazine methosulfate and 2,6-dichlorophenolindophenol as electron acceptors | Sus scrofa |