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Literature summary for 1.3.8.6 extracted from

  • Rao, K.S.; Fu, Z.; Albro, M.; Narayanan, B.; Baddam, S.; Lee, H.J.; Kim, J.J.; Frerman, F.E.
    The effect of a Glu370Asp mutation in glutaryl-CoA dehydrogenase on proton transfer to the dienolate intermediate (2007), Biochemistry, 46, 14468-14477.
    View publication on PubMed
Search for more literature for 1.3.8.6

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method with 100 mM MES buffer, pH 6.5, 30% poly(ethyleneglycol) monomethyl ether 5000, and 0.2 M ammonium sulfate, at 19°C, in complex with 4-nitrobutyric acid Homo sapiens

Protein Variants

Protein Variants Comment Organism
E370D the mutation results in a 24% decrease in the rate constant for proton abstraction at C-2 of 3-thiaglutaryl-CoA, the net rate constant for flavin reduction due to hydride transfer from C-3 of the natural substrate decreases by 81% due to the mutation Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutaryl-CoA + acceptor Homo sapiens
-
 crotonyl-CoA + CO2 + reduced acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q92947
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-thiaglutaryl-CoA + acceptor
-
 Homo sapiens ?
-
 ?
4-nitrobutyryl-CoA + acceptor
-
 Homo sapiens 4-nitro-but-2-enoyl-CoA + reduced acceptor
-
 ?
glutaconyl-CoA + ferrocenium hexafluorophosphate
-
 Homo sapiens crotonyl-CoA + CO2 + ferricenium hexafluorophosphate the kinetically favorable product is vinylacetyl-CoA, which is further isomerized to the thermodynamically stable normal product crotonyl-CoA ir
glutaryl-CoA + acceptor
-
 Homo sapiens crotonyl-CoA + CO2 + reduced acceptor
-
 ?

Synonyms

Synonyms Comment Organism
GCD
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.28
-
 glutaryl-CoA mutant enzyme E370D, at 25°C Homo sapiens
2.04
-
 glutaconyl-CoA mutant enzyme E370D, at 25°C in 50 mM potassium phosphate buffer, pH 7.6 Homo sapiens
5.5
-
 glutaconyl-CoA mutant enzyme E370D, at 25°C in 50 mM potassium phosphate buffer, pH 7.6 Homo sapiens
5.6
-
 glutaryl-CoA wild type enzyme, at 25°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Homo sapiens