Cloned (Comment) | Organism |
---|---|
expression of normal and mutant enzymes in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
L383R/R387A | has no detectable activity in crude cellular extracts | Homo sapiens |
R387A | enzyme activity detected, the mutant is less able than the mutant R387K to properly form the charge-transfer complex intermediate | Homo sapiens |
R387E | enzyme activity detected, the mutant is less able than the mutant R387K to properly form the charge-transfer complex intermediate | Homo sapiens |
R387K | enzyme activity detected, the mutant is able to form the charge-transfer complex intermediate with similar efficiency to wild-type | Homo sapiens |
R387Q | enzyme activity detected, the mutant is less able than the mutant R387K to properly form the charge-transfer complex intermediate | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
isovaleryl-CoA | wild-type | Homo sapiens | |
0.0203 | - |
isovaleryl-CoA | mutant R387K | Homo sapiens | |
0.0756 | - |
isovaleryl-CoA | mutant R387A | Homo sapiens | |
0.195 | - |
isovaleryl-CoA | mutant R387Q | Homo sapiens | |
0.9 | - |
isovaleryl-CoA | mutant R387E | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isovaleryl-CoA + acceptor | Homo sapiens | - |
3-methylcrotonyl-CoA + reduced acceptor | - |
? | |
additional information | Homo sapiens | R387 has an important role in anchoring the substrate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using bacterial lysates through centrifugation, filtration through an Acrodisc filter, chromatography on a XK16/20 DEAE fast-flow column and hydroxyapatite resin column | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isovaleryl-CoA + acceptor | - |
Homo sapiens | 3-methylcrotonyl-CoA + reduced acceptor | - |
? | |
isovaleryl-CoA + acceptor | electron acceptor: electron-transferring flavoprotein | Homo sapiens | 3-methylcrotonyl-CoA + reduced acceptor | - |
? | |
additional information | R387 has an important role in anchoring the substrate | Homo sapiens | ? | - |
? |