Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as a N-terminal histidine-tagged fusion protein | Cereibacter sphaeroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62300 | - |
2 * 62300, N-terminal histidine-tagged fusion protein, SDS-PAGE | Cereibacter sphaeroides |
100000 | - |
gel filtration | Cereibacter sphaeroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-methylsuccinyl-CoA + electron-transfer flavoprotein | Cereibacter sphaeroides | the enzyme is involved ion the ethylmalonyl-CoA pathway for acetyl-CoA assimilation | 2-methylfumaryl-CoA + reduced electron-transfer flavoprotein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | D3JV03 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Cereibacter sphaeroides |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:acetate-grown cell | - |
Cereibacter sphaeroides | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-methylsuccinyl-CoA + electron-transfer flavoprotein | the enzyme is involved ion the ethylmalonyl-CoA pathway for acetyl-CoA assimilation | Cereibacter sphaeroides | 2-methylfumaryl-CoA + reduced electron-transfer flavoprotein | - |
? | |
(2S)-methylsuccinyl-CoA + electron-transfer flavoprotein | the enzyme is highly specific for (S)-methylsuccinyl-CoA. No activity with butyryl-CoA, isobutyryl-CoA or a diastereomeric mixture of (R)-methylsuccinyl-CoA | Cereibacter sphaeroides | 2-methylfumaryl-CoA + reduced electron-transfer flavoprotein | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 62300, N-terminal histidine-tagged fusion protein, SDS-PAGE | Cereibacter sphaeroides |
Synonyms | Comment | Organism |
---|---|---|
MCD | - |
Cereibacter sphaeroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Cereibacter sphaeroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Cereibacter sphaeroides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the enzyme contains 0.7 mol of noncovalently bound FAD molecule per subunit | Cereibacter sphaeroides |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the ethylmalonyl-CoA pathway, an acetyl-CoA assimilation strategy | Cereibacter sphaeroides |