Cloned (Comment) | Organism |
---|---|
SCAD DNA and amino acid sequence determination and analysis of wild-type and mutant enzymes, coexpression iin HEK-293 cells, expression of fluorescent-labeled enzyme mutants in U2-OS cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E344G | site-directed mutagenesis, the SCAD mutant shows reduced activity compared to the wild-typ enzyme, but does not influence the wild-type SCAD activity when co-transfected in HEK-293 cells | Homo sapiens |
G108D | site-directed mutagenesis, the SCAD mutant shows reduced activity compared to the wild-typ enzyme, but does not influence the wild-type SCAD activity when co-transfected in HEK-293 cells | Homo sapiens |
P55L | site-directed mutagenesis, the SCAD mutant shows reduced activity compared to the wild-typ enzyme, but does not influence the wild-type SCAD activity when co-transfected in HEK-293 cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
butanoyl-CoA + acceptor | Homo sapiens | - |
but-2-enoyl-CoA + reduced acceptor | - |
? | |
hexanoyl-CoA + acceptor | Homo sapiens | - |
hex-2-enoyl-CoA + reduced acceptor | - |
? | |
pentanoyl-CoA + acceptor | Homo sapiens | - |
pent-2-enoyl-CoA + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | D4QEZ8 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fibroblast | - |
Homo sapiens | - |
HEK-293 cell | - |
Homo sapiens | - |
lymphocyte | - |
Homo sapiens | - |
additional information | SCAD activity levels in different tissues vary greatly, immunohistochemic analysis, overview | Homo sapiens | - |
muscle | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
butanoyl-CoA + acceptor | - |
Homo sapiens | but-2-enoyl-CoA + reduced acceptor | - |
? | |
butanoyl-CoA + FAD | - |
Homo sapiens | but-2-enoyl-CoA + FADH2 | - |
? | |
hexanoyl-CoA + acceptor | - |
Homo sapiens | hex-2-enoyl-CoA + reduced acceptor | - |
? | |
hexanoyl-CoA + FAD | - |
Homo sapiens | hex-2-enoyl-CoA + FADH2 | - |
? | |
pentanoyl-CoA + acceptor | - |
Homo sapiens | pent-2-enoyl-CoA + reduced acceptor | - |
? | |
pentanoyl-CoA + FAD | - |
Homo sapiens | pent-2-enoyl-CoA + FADH2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
butyryl-CoA dehydrogenase | - |
Homo sapiens |
SCAD | - |
Homo sapiens |
short-chain acyl-CoA dehydrogenase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
26 | 41 | activities of SCAD constructs are entirely lower at 26°C incubation temperature and slightly higher at 41°C incubation temperature than those at 37°C incubation temperature | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | mutations in the gene encodine acyl-CoA dehydrogenase, ACAD, cause alterations in SCAD activity, overview | Homo sapiens |
physiological function | SCAD is a mitochondrial enzyme involved in the beta-oxidation of fatty acids and mediates the metabolic transition from acyl-CoA with four or six carbon chains to 2-enoyl-CoA in the first step of the beta-oxidation spiral. Genetic defect of SCAD cause clinical symptoms such as progressive psychomotor retardation, muscle hypotonia, and myopathy | Homo sapiens |