Application | Comment | Organism |
---|---|---|
additional information | common variant SCAD enzymes and their potential contribution to clinical disease in humans | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
into Escherichia coli XL1 Blue | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
G185S | impairments of both its kinetic and electron transfer properties | Homo sapiens |
R147W | exhibits almost identical physical and redox properties to wild-type but only half of the specific activity and substrate activation shifts observed in wild-type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.055 | - |
butyryl-CoA | wild-type | Homo sapiens | |
0.065 | - |
butyryl-CoA | R147W mutant | Homo sapiens | |
0.124 | - |
butyryl-CoA | G185S mutant | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by gel filtration, to more than 95% purity | Homo sapiens |
Storage Stability | Organism |
---|---|
-80°C, phosphate buffer, pH 7.6, 20% glycerol | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
butyryl-CoA + electron acceptor | - |
Homo sapiens | 2-butenoyl-CoA + reduced acceptor | - |
? | |
crotonyl-CoA + electron acceptor | - |
Homo sapiens | butyryl-CoA + reduced acceptor | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SCAD | - |
Homo sapiens |
short-chain acyl-CoA dehydrogenase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Homo sapiens |