Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(methylenecyclopropyl)acetyl-CoA | 0.01 mM, 81% inhibition, 0.001 mM, 49% inhibition | Homo sapiens | |
iodoacetamide | 2 mM, 63% inhibition | Homo sapiens | |
N-ethylmaleimide | 2 mM, 41% inhibition, 0.2 mM, 23% inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0041 | - |
electron transfer flavoprotein | - |
Homo sapiens | |
0.0129 | - |
butyryl-CoA | - |
Homo sapiens | |
0.0333 | - |
pentanoyl-CoA | - |
Homo sapiens | |
0.0339 | - |
hexanoyl-CoA | - |
Homo sapiens | |
0.179 | - |
phenazine methosulfate | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41200 | - |
4 * 41200, SDS-PAGE | Homo sapiens |
168000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
butyryl-CoA + electron acceptor | intermediate electron acceptors phenazine methosulfate or electron transfer flavoprotein | Homo sapiens | 2-butenoyl-CoA + reduced acceptor | - |
? | |
butyryl-CoA + electron acceptor | terminal electron acceptor 2,6-dichlorophenol-indophenol | Homo sapiens | 2-butenoyl-CoA + reduced acceptor | - |
? | |
butyryl-CoA + electron acceptor | intermediate electron acceptor phenazine methosulfate | Homo sapiens | 2-butenoyl-CoA + reduced acceptor | - |
? | |
hexanoyl-CoA + electron transfer flavoprotein | - |
Homo sapiens | 2-hexenoyl-CoA + reduced acceptor | - |
? | |
hexanoyl-CoA + phenazine methosulfate + dichloroindophenol | - |
Homo sapiens | 2-hexenoyl-CoA + reduced acceptor | - |
? | |
pentanoyl-CoA + electron transfer flavoprotein | - |
Homo sapiens | 2-pentenoyl-CoA + reduced acceptor | - |
? | |
pentanoyl-CoA + phenazine methosulfate | pentanoyl-CoA i.e. valeryl-CoA | Homo sapiens | 2-pentenoyl-CoA + reduced acceptor | - |
? | |
propionyl-CoA + electron transfer flavoprotein | - |
Homo sapiens | 2-propenoyl-CoA + reduced acceptor | - |
? | |
propionyl-CoA + phenazine methosulfate | - |
Homo sapiens | 2-propenoyl-CoA + reduced acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 41200, SDS-PAGE | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16.5 | - |
hexanoyl-CoA | - |
Homo sapiens | |
20.8 | - |
butyryl-CoA | - |
Homo sapiens | |
40.7 | - |
valeryl-CoA | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | less than 50% of maximal activity above and below | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1 mol per mol of subunit | Homo sapiens |