Cloned (Comment) | Organism |
---|---|
expressed in Azotobacter vinelandii strain DJ884 | Cereibacter sphaeroides |
Crystallization (Comment) | Organism |
---|---|
L-protein of DPOR with Mg-ADP bound, microcapillary batch diffusion method, with 20-25% (w/v) PEG 3350 as the precipitating agent, with 200 mM magnesium formate (pH 7.7) | Cereibacter sphaeroides |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the purified, recombinant protein L contains 3.6 mol of Fe/mol of protein L homodimer, consistent with the presence of a [4Fe-4S] cluster | Cereibacter sphaeroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33310 | - |
calculated from amino acid sequence | Cereibacter sphaeroides |
33380 | - |
mass spectroscopy | Cereibacter sphaeroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | Q9RFD6 | - |
- |
Purification (Comment) | Organism |
---|---|
zinc-loaded chelating Sepharose column chromatography and Q-Sepharose column chromatography | Cereibacter sphaeroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
protochlorophyllide + oxidized ferredoxin + ADP + phosphate | - |
Cereibacter sphaeroides | chlorophyllide a + reduced ferredoxin + ATP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dark-operative protochlorophyllide reductase | - |
Cereibacter sphaeroides |
DPOR | the DPOR holoenzyme is comprised of two component proteins, the dimeric protein L and the heterotetrameric NB-protein | Cereibacter sphaeroides |
light-independent (dark) Pchlide reductase | - |
Cereibacter sphaeroides |
light-independent protochlorophyllide reductase | - |
Cereibacter sphaeroides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
4Fe-4S-center | - |
Cereibacter sphaeroides | |
ADP | - |
Cereibacter sphaeroides |