Cloned (Comment) | Organism |
---|---|
recombinant expression of GST-tagged PcyA in Escherichia coli | Anabaena sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
biliverdin IXalpha + reduced ferredoxin | Anabaena sp. | - |
phycocyanobilin + oxidized ferredoxin | with biliverdins containing a single endovinyl group, such as BV IXalpha, PcyA can generate a mixture of 3E and 3Z products | ? | |
biliverdin IXalpha + reduced ferredoxin | Anabaena sp. PCC 7120 | - |
phycocyanobilin + oxidized ferredoxin | with biliverdins containing a single endovinyl group, such as BV IXalpha, PcyA can generate a mixture of 3E and 3Z products | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anabaena sp. | - |
- |
- |
Anabaena sp. PCC 7120 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged PcyA from Escherichia coli by glutathione affinity chromatography | Anabaena sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
biliverdin IXa diamide + reduced ferredoxin | activity only at pH 6.0 | Anabaena sp. | ? + oxidized ferredoxin | - |
? | |
biliverdin IXa diamide + reduced ferredoxin | activity only at pH 6.0 | Anabaena sp. PCC 7120 | ? + oxidized ferredoxin | - |
? | |
biliverdin IXalpha + reduced ferredoxin | - |
Anabaena sp. | phycocyanobilin + oxidized ferredoxin | with biliverdins containing a single endovinyl group, such as BV IXalpha, PcyA can generate a mixture of 3E and 3Z products | ? | |
biliverdin IXalpha + reduced ferredoxin | recombinant ferredoxin from Synechococcus sp. PCC7002 | Anabaena sp. | phycocyanobilin + oxidized ferredoxin | with biliverdins containing a single endovinyl group, such as BV IXalpha, PcyA can generate a mixture of 3E and 3Z products | ? | |
biliverdin IXalpha + reduced ferredoxin | - |
Anabaena sp. PCC 7120 | phycocyanobilin + oxidized ferredoxin | with biliverdins containing a single endovinyl group, such as BV IXalpha, PcyA can generate a mixture of 3E and 3Z products | ? | |
biliverdin IXalpha + reduced ferredoxin | recombinant ferredoxin from Synechococcus sp. PCC7002 | Anabaena sp. PCC 7120 | phycocyanobilin + oxidized ferredoxin | with biliverdins containing a single endovinyl group, such as BV IXalpha, PcyA can generate a mixture of 3E and 3Z products | ? | |
biliverdin IXalpha 12-monoamide + reduced ferredoxin | - |
Anabaena sp. | phycocyanobilin 12-monoamide + oxidized ferredoxin | products are corresponding phycocyanobilin monoamides with a mix of the 3E and 3Z forms | ? | |
biliverdin IXalpha 12-monoamide + reduced ferredoxin | - |
Anabaena sp. PCC 7120 | phycocyanobilin 12-monoamide + oxidized ferredoxin | products are corresponding phycocyanobilin monoamides with a mix of the 3E and 3Z forms | ? | |
biliverdin IXalpha 8-monoamide + reduced ferredoxin | - |
Anabaena sp. | phycocyanobilin 8-monoamide + oxidized ferredoxin | products are corresponding phycocyanobilin monoamides with a mix of the 3E and 3Z forms | ? | |
biliverdin XIII + reduced ferredoxin | - |
Anabaena sp. | phycocyanobilin + oxidized ferredoxin | biliverdin XIII contains two endovinyl groups, so PcyA can convert either (or both) to an ethylidene | ? | |
biliverdin XIIIalpha monoamide + reduced ferredoxin | - |
Anabaena sp. | ? + oxidized ferredoxin | mixture of products | ? | |
additional information | semisynthesis of mono- and diamides of biliverdin IXalpha, that function as substrates. The enzyme is accommodating to amidation of the propionic acid side chains of biliverdin IXalpha, which does not require free carboxylic acid side chains to yield its phytobilin product, phycocyanobilin. Bilin amides are assembled with BV-type apophytochromes, demonstrating a role for the 8-propionate in the formation of the spectroscopically native Pr dark states of the biliprotein photosensors. Neither ionizable propionate side chain proves to be essential to primary photoisomerization for BV-type phytochrome. No activity with biliverdin XIIIalpha diamide , but biliverdinXIII diacid is converted into the iso-PthetaB product with moderate yield | Anabaena sp. | ? | - |
? | |
additional information | semisynthesis of mono- and diamides of biliverdin IXalpha, that function as substrates. The enzyme is accommodating to amidation of the propionic acid side chains of biliverdin IXalpha, which does not require free carboxylic acid side chains to yield its phytobilin product, phycocyanobilin. Bilin amides are assembled with BV-type apophytochromes, demonstrating a role for the 8-propionate in the formation of the spectroscopically native Pr dark states of the biliprotein photosensors. Neither ionizable propionate side chain proves to be essential to primary photoisomerization for BV-type phytochrome. No activity with biliverdin XIIIalpha diamide , but biliverdinXIII diacid is converted into the iso-PthetaB product with moderate yield | Anabaena sp. PCC 7120 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PcyA | - |
Anabaena sp. |
phycocyanobilin:ferredoxin oxidoreductase | - |
Anabaena sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay with substrate biliverdin diamide | Anabaena sp. |
8.5 | - |
assay at | Anabaena sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | dependent on | Anabaena sp. |
General Information | Comment | Organism |
---|---|---|
evolution | comparison and discrimination of two bilin reductase families in bilin amide usage for photoconversions of BV-type and phytobilin-type phytochromes, mechanistic differences, overview | Anabaena sp. |
additional information | the NADPH-dependent biliverdin reductases, BVR and BvdR, are less accommodating to amidation of the propionic acid side chains of biliverdin IXR than PcyA, which does not require free carboxylic acid side chains to yield its phytobilin product, phycocyanobilin | Anabaena sp. |