Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Synechococcus sp. |
expression in Escherichia coli | Gentiana lutea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
norflurazon | 0.0001 mM, Vmax values are very little influenced by norflurazon, which reveals a competition between NADP+ or plastoquinone and norflurazon for the cofactor binding site of phytoene desaturase | Synechococcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
15-cis-phytoene + 2 plastoquinone | Synechococcus sp. | - |
9,15,9'-tricis-zeta-carotene + 2 plastoquinol | - |
? | |
15-cis-phytoene + 2 plastoquinone | Gentiana lutea | - |
9,15,9'-tricis-zeta-carotene + 2 plastoquinol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gentiana lutea | - |
- |
- |
Synechococcus sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
15-cis-phytoene + 2 decyl-plastoquinone | this quinone derivative is used in the study because the natural C45-plastoquinone is not available commercially | Synechococcus sp. | 9,15,9'-tricis-zeta-carotene + 2 decyl-plastoquinol | - |
? | |
15-cis-phytoene + 2 decyl-plastoquinone | this quinone derivative is used in the study because the natural C45-plastoquinone is not available commercially. Decyl-plastoquinone is very effective as a cofactor of desaturation. The simultanous presence of NADP+ has no influence on the activity | Gentiana lutea | 9,15,9'-tricis-zeta-carotene + 2 decyl-plastoquinol | - |
? | |
15-cis-phytoene + 2 decyl-ubiquinone | this quinone derivative is used in the study because the natural C45-ubiquinone is not available commercially | Synechococcus sp. | 9,15,9'-tricis-zeta-carotene + 2 decyl-plastoquinol | - |
? | |
15-cis-phytoene + 2 decylubiquinone | this quinone derivative is used in the study because the natural C45-ubiquinone is not available commercially | Gentiana lutea | 9,15,9'-tricis-zeta-carotene + 2 decyl-plastoquinol | - |
? | |
15-cis-phytoene + 2 plastoquinone | - |
Synechococcus sp. | 9,15,9'-tricis-zeta-carotene + 2 plastoquinol | - |
? | |
15-cis-phytoene + 2 plastoquinone | - |
Gentiana lutea | 9,15,9'-tricis-zeta-carotene + 2 plastoquinol | - |
? | |
additional information | no activity with NADP+ | Gentiana lutea | ? | - |
? | |
additional information | the enzyme also shows activity with NADP+ as cosubstrate, the affinity for NADP+ is lower than for decylplastoquinone or decylubioquinone | Synechococcus sp. | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Synechococcus sp. |
28 | - |
assay at | Gentiana lutea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Synechococcus sp. |
7.2 | - |
assay at | Gentiana lutea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
decyl-plastoquinone | this quinone derivative is used in the study because the natural C45-plastoquinone is not available commercially | Gentiana lutea | |
additional information | the enzyme also shows activity with NADP+ as cosubstrate, the affinity for NADP+ is lower than for decylplastoquinone or decylubioquinone | Synechococcus sp. | |
plastoquinone | decyl-plastoquinone or decyl-ubiquinone is used in the study because the natural C45-plastoquinone is not available commercially. The affinity of the enzyme for plastoquinone is higher than for NADP+ | Synechococcus sp. | |
Ubiquinone-10 | this quinone derivative is used in the study because the natural C45-plastoquinone is not available commercially | Gentiana lutea |