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Literature summary for 1.3.5.3 extracted from
- Identification of Escherichia coli HemG as a novel, menadione-dependent flavodoxin with protoporphyrinogen oxidase activity (2009), Biochemistry, 48, 6705-6711.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | Escherichia coli strain SASX38 lacks functional protoporphyrinogen IX dehydrogenase activity and is reported to be deficient in HemG. Sequence analysis of the SASX38 hemG gene reveals a deletion from nucleotide 152 to 470. This results in a predicted HemG protein that remains in frame but lacks 106 internal amino acids, including the long chain insert loop | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00376 | - |
menadione | pH 8.0, 37°C | Escherichia coli |  |
| 0.007 | - |
protoporphyrinogen IX | pH 8.0, 37°C | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22000 | - |
4 * 22000, SDS-PAGE | Escherichia coli |
| 22500 | - |
4 * 22500, calculated from sequence | Escherichia coli |
| 108000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protoporphyrinogen IX + 3 menadione | Escherichia coli | the enzyme enables Escherichia coli to synthesize heme in both aerobic and anaerobic environments | protoporphyrin IX + 3 menadiol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| six histidine-tagged recombinant protein | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protoporphyrinogen IX + 3 menadione | the enzyme enables Escherichia coli to synthesize heme in both aerobic and anaerobic environments | Escherichia coli | protoporphyrin IX + 3 menadiol | - |
? | |
| protoporphyrinogen IX + 3 menadione | HemG is specific for protoporphyrinogen. Neither mesoporphyrinogen nor coproporphyrinogen is oxidized by HemG | Escherichia coli | protoporphyrin IX + 3 menadiol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 22000, SDS-PAGE | Escherichia coli |
| tetramer | 4 * 22500, calculated from sequence | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HemG protein | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.281 | - |
menadione | pH 8.0, 37°C | Escherichia coli | |
| 0.292 | - |
protoporphyrinogen IX | pH 8.0, 37°C | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | contains FMN | Escherichia coli | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 41.7 | - |
protoporphyrinogen IX | pH 8.0, 37°C | Escherichia coli | |
| 74.73 | - |
menadione | pH 8.0, 37°C | Escherichia coli | |
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