Any feedback?
Literature summary for 1.3.5.2 extracted from
- Mechanism of flavin reduction in class 2 dihydroorotate dehydrogenases (2006), Biochemistry, 45, 14926-14932.
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | kinetic isotope effects on flavin reduction in anaerobic stopped-flow experiments, are about 3fold for DHO labeled at the 5-position, about 4fold for DHO labeled at the 6-position, and about 6-7fold for DHO labeled at both the 5- and 6-positions, at a pH value above the pKa controlling reduction, no isotope effect was observed for DHO deuterated at the 5-position, which is consistent with a stepwise reaction, above the kinetic pKa, the deprotonation of C5 is fast enough that it does not contribute to the observed rate constant and, therefore, is not isotopically sensitive | Escherichia coli |
| additional information | kinetic isotope effects on flavin reduction in anaerobic stopped-flow experiments, pKa near 9.4 controlling reduction, similar to that previously reported for the Escherichia coli enzyme | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
- |
Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydroorotate + acceptor | - |
Escherichia coli | orotate + reduced acceptor | - |
? |  |
| dihydroorotate + acceptor | - |
Homo sapiens | orotate + reduced acceptor | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| class 2 dihydroorotate dehydrogenases | - |
Escherichia coli |
| class 2 dihydroorotate dehydrogenases | - |
Homo sapiens |
| DHODH | - |
Escherichia coli |
| DHODH | - |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11.3 | the rate constant for flavin reduction increases with pH | Homo sapiens |
| 6.5 | 8.5 | - |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| flavin | - |
Escherichia coli | |
| flavin | - |
Homo sapiens | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
