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Literature summary for 1.3.5.2 extracted from

  • Baldwin, J.; Farajallah, A.M.; Malmquist, N.A.; Rathod, P.K.; Phillips, M.A.
    Malarial dihydroorotate dehydrogenase. Substrate and inhibitor specificity (2002), J. Biol. Chem., 277, 41827-41834.
    View publication on PubMed
Search for more literature for 1.3.5.2

Cloned(Commentary)

Cloned (Comment) Organism
-
 Plasmodium falciparum

Protein Variants

Protein Variants Comment Organism
additional information N-terminally truncated protein lacking 168 amino acids Plasmodium falciparum
additional information N-terminally truncated protein lacking first 29 amino acids Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2-cyano-3-cyclopropyl-3-hydroxy-N-[4'-(cyano)phenyl]-propenamide 50% inhibition at 0.0.00035 mM, N-terminally truncated protein Homo sapiens
2-cyano-3-cyclopropyl-3-hydroxy-N-[4'-(cyano)phenyl]-propenamide 50% inhibition at 0.712 mM, N-terminally truncated protein Plasmodium falciparum
2-cyano-3-cyclopropyl-3-hydroxy-N-[4'-(nitro)phenyl]-propenamide 50% inhibition at 0.00025 mM, N-terminally truncated protein Homo sapiens
2-cyano-3-cyclopropyl-3-hydroxy-N-[4'-(nitro)phenyl]-propenamide 50% inhibition at 0.543 mM, N-terminally truncated protein Plasmodium falciparum
2-cyano-3-cyclopropyl-3-hydroxy-N-[4'-(trifluoromethyl)phenyl]-propenamide 50% inhibition at 0.00019 mM, N-terminally truncated protein Homo sapiens
2-cyano-3-cyclopropyl-3-hydroxy-N-[4'-(trifluoromethyl)phenyl]-propenamide 50% inhibition at 1.08 mM, N-terminally truncated protein Plasmodium falciparum
3-hydroxy-2-(3,3-dichloroallyl)-1,4-naphthoquinone 50% inhibition at 0.000065 mM, i. e. dichloroallyl lawsone, N-terminally truncated protein Homo sapiens
3-hydroxy-2-(3,3-dichloroallyl)-1,4-naphthoquinone 50% inhibition at 0.220 mM, i. e. dichloroallyl lawsone, N-terminally truncated protein Plasmodium falciparum
redoxal 50% inhibition at 0.000013 mM, N-terminally truncated protein Homo sapiens
redoxal 50% inhibition at 0.071 mM, N-terminally truncated protein Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 L-dihydroorotate pH 8.0, 25°C, cosubstrate Q6, N-terminally truncated protein Plasmodium falciparum
0.016
-
 L-dihydroorotate pH 8.0, 25°C, cosubstrate QD, N-terminally truncated protein Plasmodium falciparum
0.021
-
 L-dihydroorotate pH 8.0, 25°C, cosubstrate Q4, N-terminally truncated protein Plasmodium falciparum
0.032
-
 decylubiquinone pH 8.0, 25°C, cosubstrate L-dihydroorotate, N-terminally truncated protein Plasmodium falciparum
0.035
-
 ubiquinone-4 pH 8.0, 25°C, cosubstrate L-dihydroorotate, N-terminally truncated protein Plasmodium falciparum
0.056
-
 L-dihydroorotate pH 8.0, 25°C, cosubstrate vitamin K3, N-terminally truncated protein Plasmodium falciparum
0.062
-
 ubiquinone-6 pH 8.0, 25°C, cosubstrate L-dihydroorotate, N-terminally truncated protein Plasmodium falciparum
0.114
-
 L-dihydroorotate pH 8.0, 25°C, cosubstrate Q0, N-terminally truncated protein Plasmodium falciparum

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion outer membrane of Plasmodium falciparum 5739
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 recombinant truncated enzyme
-
Plasmodium falciparum Q08210 expression in Escherichia coli
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-dihydroorotate + decylubiquinone
-
 Plasmodium falciparum orotate + reduced decylubiquinone
-
 ?
L-dihydroorotate + ubiquinone-0
-
 Plasmodium falciparum orotate + reduced ubiquinone-0
-
 ?
L-dihydroorotate + ubiquinone-4
-
 Plasmodium falciparum orotate + reduced ubiquinone-4
-
 ?
L-dihydroorotate + ubiquinone-6
-
 Plasmodium falciparum orotate + reduced ubiquinone-6
-
 ?
L-dihydroorotate + vitamin K3
-
 Plasmodium falciparum orotate + reduced vitamin K3
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8
-
 decylubiquinone pH 8.0, 25°C, cosubstrate L-dihydroorotate, N-terminally truncated protein Plasmodium falciparum
1.8
-
 ubiquinone-4 pH 8.0, 25°C, cosubstrate L-dihydroorotate, N-terminally truncated protein Plasmodium falciparum
1.8
-
 L-dihydroorotate pH 8.0, 25°C, cosubstrate Q0, N-terminally truncated protein Plasmodium falciparum
2
-
 ubiquinone-6 pH 8.0, 25°C, cosubstrate L-dihydroorotate, N-terminally truncated protein Plasmodium falciparum
2
-
 L-dihydroorotate pH 8.0, 25°C, cosubstrate Q4, N-terminally truncated protein Plasmodium falciparum
2
-
 L-dihydroorotate pH 8.0, 25°C, cosubstrate Q6, N-terminally truncated protein Plasmodium falciparum
2
-
 L-dihydroorotate pH 8.0, 25°C, cosubstrate QD, N-terminally truncated protein Plasmodium falciparum
2
-
 L-dihydroorotate pH 8.0, 25°C, cosubstrate vitamin K3, N-terminally truncated protein Plasmodium falciparum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 N-terminally truncated protein Plasmodium falciparum

Cofactor

Cofactor Comment Organism Structure
2-methyl-1,4-naphthoquinone little catalytic efficiency, N-terminally truncated protein Plasmodium falciparum
FMN 0.9 molar equivalents, N-terminally truncated protein Plasmodium falciparum
ubiquinone-0 little catalytic efficiency, N-terminally truncated protein Plasmodium falciparum
Ubiquinone-10 CoQD, efficient, N-terminally truncated protein Plasmodium falciparum
ubiquinone-4 efficient, N-terminally truncated protein Plasmodium falciparum
ubiquinone-8 efficient, N-terminally truncated protein Plasmodium falciparum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.059
-
 redoxal pH 8.0, 25°C, varied substrate L-dihydroorotate, N-terminally truncated protein Plasmodium falciparum
0.086
-
 redoxal pH 8.0, 25°C, varied substrate QD, N-terminally truncated protein Plasmodium falciparum
0.16
-
 3-hydroxy-2-(3,3-dichloroallyl)-1,4-naphthoquinone pH 8.0, 25°C, varied substrate L-dihydroorotate, N-terminally truncated protein Plasmodium falciparum
0.25
-
 3-hydroxy-2-(3,3-dichloroallyl)-1,4-naphthoquinone pH 8.0, 25°C, varied substrate QD, N-terminally truncated protein Plasmodium falciparum
0.4
-
 2-cyano-3-cyclopropyl-3-hydroxy-N-[4'-(nitro)phenyl]-propenamide pH 8.0, 25°C, varied substrate QD, N-terminally truncated protein Plasmodium falciparum