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Literature summary for 1.3.5.2 extracted from

  • Ullrich, A.; Knecht, W.; Piskur, J.; Loffler, M.
    Plant dihydroorotate dehydrogenase differs significantly in substrate specificity and inhibition from the animal enzymes (2002), FEBS Lett., 529, 346-350.
    View publication on PubMed
Search for more literature for 1.3.5.2

Protein Variants

Protein Variants Comment Organism
DELTA1-75 N-terminally truncated enzyme, drastic increase in kcat- and KM-value Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 dihydroorotate pH 8.0, 30°C, wild-type Arabidopsis thaliana
0.112
-
 decylubiquinone pH 8.0, 30°C, wild-type Arabidopsis thaliana
0.121
-
 dihydroorotate pH 8.0, 30°C, DELTA1-75 mutant Arabidopsis thaliana
0.341
-
 decylubiquinone pH 8.0, 30°C, DELTA1-75 mutant Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48500
-
 x * 48500, deduced from gene sequendce Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P32746
-
-

Reaction

Reaction Comment Organism Reaction ID
(S)-dihydroorotate + a quinone = orotate + a quinol two-site ping-pong mechanism Arabidopsis thaliana
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydroorotate + 2,5-dimethyl-p-benzoquinone 95% the rate of decylubiquinone, wild-type, 75% the rate of decylubiquinone, DELTA1-75 mutant Arabidopsis thaliana orotate + reduced 2,5-dimethyl-p-benzoquinone
-
 ?
dihydroorotate + CoQ0 102% the rate of decylubiquinone, wild-type, 67% the rate of decylubiquinone, DELTA1-75 mutant Arabidopsis thaliana orotate + reduced CoQo
-
 ?
dihydroorotate + decylplastoquinone 95% the rate of decylubiquinone, wild-type, 104% the rate of decylubiquinone, DELTA1-75 mutant Arabidopsis thaliana orotate + reduced decylplastoquinone
-
 ?
dihydroorotate + decylubiquinone
-
 Arabidopsis thaliana orotate + reduced decylubiquinone
-
 ?
dihydroorotate + menadione 82% the rate of decylubiquinone, wild-type, 78% the rate of decylubiquinone, DELTA1-75 mutant Arabidopsis thaliana orotate + reduced menadione
-
 ?
dihydroorotate + ubiquinone-50 83% the rate of decylubiquinone, wild-type, 76% the rate of decylubiquinone, DELTA1-75 mutant Arabidopsis thaliana orotate + reduced ubiquinone-50
-
 ?

Subunits

Subunits Comment Organism
? x * 48500, deduced from gene sequendce Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.2
-
 dihydroorotate pH 8.0, 30°C, wild-type Arabidopsis thaliana
72.9
-
 dihydroorotate pH 8.0, 30°C, DELTA1-75 mutant Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
flavin 0.8-1.1 mol per mol of protein Arabidopsis thaliana