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Literature summary for 1.3.5.2 extracted from
- Expression, purification, and characterization of histidine-tagged rat and human flavoenzyme dihydroorotate dehydrogenase (1998), Protein Expr. Purif., 13, 414-422.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | - |
Homo sapiens |
| medicine | - |
Rattus norvegicus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli XL-1 Blue, histidine tagged | Homo sapiens |
| expressed in Escherichia coli XL-1 Blue, histidine tagged | Rattus norvegicus |
| expression of complete catalytically active enzyme in SF21 insect cells of Spodoptera frugiperda with baculovirus vector system | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-hydroxyethylidene-cyanoacetic acid 4-trifluoromethyl anilide | inbibits by interference with the transfer of electrons from flavin to quinone | Homo sapiens |  |
| A77 1726 | - |
Homo sapiens | |
| A77 1726 | - |
Rattus norvegicus | |
| brequinar sodium | inbibits by interference with the transfer of electrons from flavin to quinone | Homo sapiens | |
| brequinar sodium | - |
Rattus norvegicus | |
| leflunomide | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0062 | - |
S-dihydroorotate | expressed in Escherichia coli | Homo sapiens | |
| 0.0065 | - |
decylubiquinone | expressed in SF21 insect cells | Rattus norvegicus | |
| 0.0095 | - |
decyclubiquinone | recombinant enzyme | Rattus norvegicus | |
| 0.0095 | - |
decylubiquinone | expressed in Escherichia coli | Rattus norvegicus | |
| 0.0135 | - |
S-dihydroorotate | expressed in SF21 insect cells | Rattus norvegicus | |
| 0.0137 | - |
decylubiquinone | expressed in Escherichia coli | Homo sapiens | |
| 0.0146 | - |
S-dihydroorotate | expressed in Escherichia coli | Rattus norvegicus | |
| 0.015 | - |
dihydroorotate | recombinant enzyme | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Homo sapiens | 5739 | - |
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
x * 45100, SDS-PAGE, recombinant from SF21 insect cells, x * 45000, SDS-PAGE, recombinant from Escherichia coli | Rattus norvegicus |
| 45100 | - |
x * 45100, SDS-PAGE, recombinant from SF21 insect cells, x * 45000, SDS-PAGE, recombinant from Escherichia coli | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-dihydroorotate + ubiquinone | Homo sapiens | six-step biosynthesis of pyrimidine uridine monophosphate | orotate + ubiquinol | - |
? | |
| (S)-dihydroorotate + ubiquinone | Rattus norvegicus | six-step biosynthesis of pyrimidine uridine monophosphate | orotate + ubiquinol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Homo sapiens | - |
recombinant protein | - |
| Rattus norvegicus | - |
- |
- |
| Rattus norvegicus | - |
recombinant protein | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+/nitrilotriacetate column | Homo sapiens |
| Ni2+/nitrilotriacetate column | Rattus norvegicus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 83 | - |
expressed in Escherichia coli | Rattus norvegicus |
| 130 | - |
expressed in SF21 insect cells | Rattus norvegicus |
| 130 | 150 | - |
Rattus norvegicus |
| 150 | - |
- |
Homo sapiens |
| 150 | - |
expressed in Escherichia coli | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-dihydroorotate + decylubiquinone | - |
Rattus norvegicus | orotate + decylubiquinol | - |
r | |
| (S)-dihydroorotate + ubiquinone | - |
Rattus norvegicus | orotate + ubiquinol | - |
? | |
| (S)-dihydroorotate + ubiquinone | decylubiquinone as electron acceptor | Homo sapiens | orotate + ubiquinol | - |
? | |
| (S)-dihydroorotate + ubiquinone | six-step biosynthesis of pyrimidine uridine monophosphate | Homo sapiens | orotate + ubiquinol | - |
? | |
| (S)-dihydroorotate + ubiquinone | six-step biosynthesis of pyrimidine uridine monophosphate | Rattus norvegicus | orotate + ubiquinol | - |
? | |
| dihydroorotate + acceptor | - |
Homo sapiens | orotate + reduced acceptor | - |
? | |
| dihydroorotate + acceptor | - |
Rattus norvegicus | orotate + reduced acceptor | - |
? | |
| L-dihydroorotate + decylubiquinone | - |
Homo sapiens | orotate + reduced decylubiquinone | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | - |
Homo sapiens |
| ? | x * 45100, SDS-PAGE, recombinant from SF21 insect cells, x * 45000, SDS-PAGE, recombinant from Escherichia coli | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Homo sapiens |
| 8 | - |
- |
Rattus norvegicus |
| 8 | 8.1 | - |
Homo sapiens |
| 8 | 8.1 | - |
Rattus norvegicus |
| 8 | 8.1 | recombinant enzymes with His-tags | Homo sapiens |
| 8 | 8.1 | recombinant enzymes with His-tags | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| flavin | - |
Rattus norvegicus | |
| FMN | 0.5-0.8 mol/mol | Homo sapiens | |
| FMN | 0.8-1.1 mol/mol | Rattus norvegicus | |
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