Cloned (Comment) | Organism |
---|---|
recombinant expression of C-terminally His6-tagged or N-termminally His8-tagged enzyme in Thermus thermophilus under the control of a constitutive promoter | Thermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-nitropropionic acid | - |
Thermus thermophilus | |
malonate | - |
Thermus thermophilus | |
nonyl-4-hydroxyquinoline-N-oxide | the semiquinone analog and inhibitor of quinone reactions in complex II shows no influence on the redox behavior of the heme b moieties | Thermus thermophilus | |
oxaloacetate | - |
Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | interprotomer temperature-dependent positive cooperativity in the trimeric complex. Only the trimer, not the monomer, exhibits positive cooperativity at high temperatures | Thermus thermophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Thermus thermophilus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the enzyme contains a [3Fe-4S] center | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
119800 | - |
monomeric recombinant C-terminally His6-tagged enzyme, gel filtration | Thermus thermophilus |
120000 | - |
1 * 120000, about, recombinant C-terminally His6-tagged enzyme, SDS-PAGE, trimerization is disrupted in rcIISdhB-His6 due to the insertion of a hexahistidine tag on the C-terminus of SdhB subunit and the resulting protein complex can only form a monomer | Thermus thermophilus |
120000 | - |
3 * 120000, about, wild-type enzyme, SDS-PAGE | Thermus thermophilus |
120000 | - |
4 * 120000, about, recombinant N-terminally His8-tagged enzyme, SDS-PAGE, four subunits of the rcII-His8-SdhB complex | Thermus thermophilus |
500000 | - |
wild-type enzyme, consisting of 360 kDa from protein and an unknown contribution of detergent and lipid, native PAGE | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinate + menaquinone | Thermus thermophilus | - |
fumarate + menaquinol | - |
? | |
succinate + menaquinone | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
fumarate + menaquinol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6- or His8-tagged enzyme from Thermus thermophilus by nickel affinity chromatography and gel filtration, untagged enzyme by anion exchange chromatography and gel filtration | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinate + menaquinone | - |
Thermus thermophilus | fumarate + menaquinol | - |
? | |
succinate + menaquinone | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | fumarate + menaquinol | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 120000, about, recombinant C-terminally His6-tagged enzyme, SDS-PAGE, trimerization is disrupted in rcIISdhB-His6 due to the insertion of a hexahistidine tag on the C-terminus of SdhB subunit and the resulting protein complex can only form a monomer | Thermus thermophilus |
tetramer | 4 * 120000, about, recombinant N-terminally His8-tagged enzyme, SDS-PAGE, four subunits of the rcII-His8-SdhB complex | Thermus thermophilus |
trimer | 3 * 120000, about, wild-type enzyme, SDS-PAGE | Thermus thermophilus |
Synonyms | Comment | Organism |
---|---|---|
SQR | - |
Thermus thermophilus |
succinate:quinone reductase | - |
Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | covalently-bound flavin cofactor | Thermus thermophilus | |
heme | the enzyme contains two heme b cofactors, a di-heme | Thermus thermophilus |
General Information | Comment | Organism |
---|---|---|
physiological function | succinate:quinone reductase serves as the respiratory complex II | Thermus thermophilus |