Literature summary for 1.3.5.1 extracted from

Garcia, L.M.; Contreras-Zentella, M.L.; Jaramillo, R.; Benito-Mercade, M.C.; Mendoza-Hernandez, G.; del Arenal, I.P.; Membrillo-Hernandez, J.; Escamilla, J.E.
The succinate:menaquinone reductase of Bacillus cereus: characterization of the membrane-bound and purified enzyme (2008), Can. J. Microbiol., 54, 456-466.

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Inhibitors

Inhibitors	Comment	Organism	Structure
2-n-heptyl-4-hydroxyquinolino-N-oxide	-	Bacillus cereus
malonate	competitive	Bacillus cereus
additional information	not inhibitory: thenoyltrifluoroacetone, 2-n-heptyl-4-hydroxyquinoline N-oxide	Bacillus cereus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.011	-	decylubiquinone	pH 7.6, 30°C	Bacillus cereus
0.33	-	phenazine methosulfate	pH 7.6, 30°C	Bacillus cereus
0.64	-	succinate	pH 7.6, 30°C	Bacillus cereus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Bacillus cereus	16020	-

Organism

Organism	UniProt	Comment	Textmining
Bacillus cereus	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
succinate + a quinone = fumarate + a quinol	reduction of menaquinone-7 is the rate-limiting step in succinate respiration	Bacillus cereus	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0076	-	electron accepotr 2,6-dichlorophenolindophenol, pH 7.6, 30°C	Bacillus cereus
0.0076	-	electron acceptor 2,6-dichlorophenolindophenol, pH 7.6, 30°C	Bacillus cereus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
fumarate + reduced decylubiquinone	-	Bacillus cereus	succinate + decylubiquinone	-	r
additional information	enzyme also accepts reduced decylubiquinone, reaction of EC 1.3.5.1	Bacillus cereus	?	-	?
additional information	enzyme also accetps phenazine methosulfate, reaction of EC 1.3.5.4	Bacillus cereus	?	-	?
succinate + decylubiquinone	-	Bacillus cereus	fumarate + reduced decylubiquinone	-	r
succinate + phenazine methosulfate	-	Bacillus cereus	fumarate + reduced phenazine methosulfate	-	r

Subunits

Subunits	Comment	Organism
?	enzyme consists of a 65000 Da flavoprotein SdhA, a 29000 Da iron-sulfur protein SdhB, and a 19000 Da subunit SdhC containing two b-type cytochromes, SDS-PAGE	Bacillus cereus

Synonyms

Synonyms	Comment	Organism
SQR	-	Bacillus cereus
SQR	ambiguous	Bacillus cereus
succinate:menaquinone 7-reductase	-	Bacillus cereus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	oxidation of succinate, assay at	Bacillus cereus
30	-	reduction of fumarate, assay at	Bacillus cereus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
77	-	phenazine methosulfate	pH 7.6, 30°C	Bacillus cereus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.8	-	with decylubiquinone-dichlorophenolindophenol as electron acceptor	Bacillus cereus
7.8	-	with phenazine methosulfate-dichlorophenolindophenyl as electron acceptor	Bacillus cereus

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Bacillus cereus
heme	-	Bacillus cereus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00062	-	2-n-heptyl-4-hydroxyquinolino-N-oxide	pH 7.6, 30°C	Bacillus cereus
0.088	-	malonate	pH 7.6, 30°C	Bacillus cereus

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Release 2023.1 (January 2023)