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Literature summary for 1.3.3.6 extracted from

  • Sztajer, H.; Wagner, G.; Schmid, R.D.
    Bacterial short-chain acyl-CoA oxidase: production, purification and characterization (1993), Appl. Microbiol. Biotechnol., 39, 708-713.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
MnCl2 slightly activating Glutamicibacter nicotianae

Inhibitors

Inhibitors Comment Organism Structure
AgNO3
-
Glutamicibacter nicotianae
HgCl2
-
Glutamicibacter nicotianae
p-chloromercuribenzoate slight inhibition Glutamicibacter nicotianae
Phenylmethylsulfonylfluoride slight inhibition Glutamicibacter nicotianae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.061
-
octanoyl-CoA
-
Glutamicibacter nicotianae
0.092
-
hexanoyl-CoA
-
Glutamicibacter nicotianae
0.1319
-
butyryl-CoA
-
Glutamicibacter nicotianae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
2 * 50000, SDS-PAGE Glutamicibacter nicotianae
100000
-
gel filtration Glutamicibacter nicotianae

Organism

Organism UniProt Comment Textmining
Glutamicibacter nicotianae
-
inducible
-

Purification (Commentary)

Purification (Comment) Organism
-
Glutamicibacter nicotianae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
77.14
-
purified enzyme Glutamicibacter nicotianae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + O2 highly specific for short-chain fatty acids Glutamicibacter nicotianae trans-2,3-dehydroacyl-CoA + H2O2
-
?
butyryl-CoA + O2
-
Glutamicibacter nicotianae trans-2-butenoyl-CoA + H2O2
-
?
hexanoyl-CoA + O2
-
Glutamicibacter nicotianae (2E)-hex-2-enoyl-CoA + H2O2
-
?
octanoyl-CoA + O2
-
Glutamicibacter nicotianae trans-2-octenoyl-CoA + H2O2
-
?

Subunits

Subunits Comment Organism
dimer 2 * 50000, SDS-PAGE Glutamicibacter nicotianae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
FAD enhances thermal stability Glutamicibacter nicotianae
60
-
30 min, with butyryl-CoA and FAD, 50% loss of activity Glutamicibacter nicotianae
60
-
30 min, with butyryl-CoA, without FAD, 85% loss of activity Glutamicibacter nicotianae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.07
-
octanoyl-CoA
-
Glutamicibacter nicotianae
0.203
-
hexanoyl-CoA
-
Glutamicibacter nicotianae
0.225
-
butyryl-CoA
-
Glutamicibacter nicotianae

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Glutamicibacter nicotianae