Activating Compound | Comment | Organism | Structure |
---|---|---|---|
MnCl2 | slightly activating | Glutamicibacter nicotianae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | - |
Glutamicibacter nicotianae | |
HgCl2 | - |
Glutamicibacter nicotianae | |
p-chloromercuribenzoate | slight inhibition | Glutamicibacter nicotianae | |
Phenylmethylsulfonylfluoride | slight inhibition | Glutamicibacter nicotianae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.061 | - |
octanoyl-CoA | - |
Glutamicibacter nicotianae | |
0.092 | - |
hexanoyl-CoA | - |
Glutamicibacter nicotianae | |
0.1319 | - |
butyryl-CoA | - |
Glutamicibacter nicotianae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, SDS-PAGE | Glutamicibacter nicotianae |
100000 | - |
gel filtration | Glutamicibacter nicotianae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glutamicibacter nicotianae | - |
inducible | - |
Purification (Comment) | Organism |
---|---|
- |
Glutamicibacter nicotianae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
77.14 | - |
purified enzyme | Glutamicibacter nicotianae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + O2 | highly specific for short-chain fatty acids | Glutamicibacter nicotianae | trans-2,3-dehydroacyl-CoA + H2O2 | - |
? | |
butyryl-CoA + O2 | - |
Glutamicibacter nicotianae | trans-2-butenoyl-CoA + H2O2 | - |
? | |
hexanoyl-CoA + O2 | - |
Glutamicibacter nicotianae | (2E)-hex-2-enoyl-CoA + H2O2 | - |
? | |
octanoyl-CoA + O2 | - |
Glutamicibacter nicotianae | trans-2-octenoyl-CoA + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, SDS-PAGE | Glutamicibacter nicotianae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
FAD enhances thermal stability | Glutamicibacter nicotianae |
60 | - |
30 min, with butyryl-CoA and FAD, 50% loss of activity | Glutamicibacter nicotianae |
60 | - |
30 min, with butyryl-CoA, without FAD, 85% loss of activity | Glutamicibacter nicotianae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
octanoyl-CoA | - |
Glutamicibacter nicotianae | |
0.203 | - |
hexanoyl-CoA | - |
Glutamicibacter nicotianae | |
0.225 | - |
butyryl-CoA | - |
Glutamicibacter nicotianae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein | Glutamicibacter nicotianae |