Application | Comment | Organism |
---|---|---|
diagnostics | the BOD activity catalyzing the oxidation of bilirubin to biliverdin can be used for the diagnosis of jaundice and hyperbilirubinemia | Bacillus pumilus |
energy production | the BOD from Bacillus pumilus is an attractive candidate for application in biofuel cells and biosensors showing high activity at neutral pH and high tolerance towards NaCl | Bacillus pumilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
bilirubin | unconjugated bilirubin, pH 7.2, 37°C | Bacillus pumilus | |
0.0351 | - |
bilirubin | conjugated bilirubin, pH 7.2, 37°C | Bacillus pumilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | the enzyme is a multicopper oxidase, MCO, whhich contains 4 copper ions per enzyme molecule | Bacillus pumilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35000, folded enzyme, SDS-PAGE, x * 60000-65000, heat denatured, unfolded enzyme, SDS-PAGE, x * 61006, mass spectrometry | Bacillus pumilus |
61006 | - |
x * 35000, folded enzyme, SDS-PAGE, x * 60000-65000, heat denatured, unfolded enzyme, SDS-PAGE, x * 61006, mass spectrometry | Bacillus pumilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 bilirubin + O2 | Bacillus pumilus | the enzyme from Bacillus pumilus shows higher turnover activity towards bilirubin compared to other bacterial MCOs | 2 biliverdin + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus pumilus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 bilirubin + O2 | the enzyme from Bacillus pumilus shows higher turnover activity towards bilirubin compared to other bacterial MCOs | Bacillus pumilus | 2 biliverdin + 2 H2O | - |
? | |
2 bilirubin + O2 | substrates are unconjugated bilirubin and conjugated bilirubin | Bacillus pumilus | 2 biliverdin + 2 H2O | - |
? | |
additional information | the enzyme is also activity with the laccase, EC 1.10.3.2, substrates 2, 2'-azino-bis (3-ethylbenzthiazoline-6-sulfonate), syringaldazine and 2,6-dimethoxyphenol | Bacillus pumilus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, folded enzyme, SDS-PAGE, x * 60000-65000, heat denatured, unfolded enzyme, SDS-PAGE, x * 61006, mass spectrometry | Bacillus pumilus |
Synonyms | Comment | Organism |
---|---|---|
BOD | - |
Bacillus pumilus |
CotA | - |
Bacillus pumilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
assay with substrate syringaldazine at 25°C | Bacillus pumilus |
37 | - |
assay at, with bilirubin as substrate | Bacillus pumilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
purified enzyme, pH 7.0, no activity decrease after preincubation for 300 min | Bacillus pumilus |
80 | - |
purified enzyme, pH 7.0, less than 50% loss in activity after preincubation for 90 min | Bacillus pumilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
66.8 | - |
bilirubin | conjugated bilirubin, pH 7.2, 37°C | Bacillus pumilus | |
70 | - |
bilirubin | unconjugated bilirubin, pH 7.2, 37°C | Bacillus pumilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
assay with substrates 2, 2'-azino-bis (3-ethylbenzthiazoline-6-sulfonate), syringaldazine and 2,6-dimethoxyphenol at pH 3.2, pH 6.2, and pH 6.8, respectively | Bacillus pumilus |
7.2 | - |
assay at, with bilirubin as substrate, the oxidation of unconjugated bilirubin can only be measured for pH above 7.0 | Bacillus pumilus |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a CotA multicopper oxidase, MCO | Bacillus pumilus |