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Literature summary for 1.3.3.4 extracted from

  • Yang, K.; Jung, S.; Lee, Y.; Back, K.
    Modifying Myxococcus xanthus protoporphyrinogen oxidase to plant codon usage and high level of oxyfluorfen resistance in transgenic rice (2006), Pestic. Biochem. Physiol., 86, 186-194.
No PubMed abstract available

Application

Application Comment Organism
additional information in rice plants, outstanding resistance to Protox-inhibiting herbicides can be achieved by expression of Myxococcus xanthus Protox Myxococcus xanthus
additional information in rice plants, outstanding resistance to Protox-inhibiting herbicides can be achieved by expression of Myxococcus xanthus Protox synthetic construct

Cloned(Commentary)

Cloned (Comment) Organism
cloned into plasmid pET28(b), expression in Escherichia coli BL21(DE3), subcloned into pGA1611 under the control of constitutive maize ubiquitin promoter and expressed in transgenic rice plant lines S11 and S19 synthetic construct
expression in transgenic rice plant lines M4 and and M7 Myxococcus xanthus

Protein Variants

Protein Variants Comment Organism
additional information construction of a synthetic Myxococcus xanthus Protox gene, with a sequence optimized to more closely match the codon usage and G+C content of the Arabidopsis Protox gene, decreased G+C content to 49% similar to that of Arapbidopsis synthetic construct

Inhibitors

Inhibitors Comment Organism Structure
oxyfuorfen all wild-type and transgenic rice lines have a significant decrease in Protox activity, all transgenic rice lines expressing native Protox show enhanced resistance to oxyfluorfen as compared to nontransgenic rice Myxococcus xanthus
oxyfuorfen all wild-type and transgenic rice lines have a significant decrease in Protox activity, all transgenic rice lines expressing modified Protox show enhanced resistance to oxyfluorfen as compared to nontransgenic rice synthetic construct

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Myxococcus xanthus 9507
-
chloroplast
-
synthetic construct 9507
-
mitochondrion
-
Myxococcus xanthus 5739
-
mitochondrion
-
synthetic construct 5739
-

Organism

Organism UniProt Comment Textmining
Myxococcus xanthus P56601
-
-
synthetic construct AY916795
-
-

Purification (Commentary)

Purification (Comment) Organism
by affinity chromatography synthetic construct

Storage Stability

Storage Stability Organism
-20°C, Tris-HCl buffer, pH 7.0, 50% glycerol Myxococcus xanthus
-20°C, Tris–HCl buffer, pH 7.0, 50% glycerol synthetic construct

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
protoporphyrinogen-IX + 3 O2
-
Myxococcus xanthus protoporphyrin-IX + 3 H2O2
-
?
protoporphyrinogen-IX + 3 O2
-
synthetic construct protoporphyrin-IX + 3 H2O2
-
?

Synonyms

Synonyms Comment Organism
protox
-
Myxococcus xanthus
protox
-
synthetic construct