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Literature summary for 1.3.3.4 extracted from
- Cloning and characterization of the yeast HEM14 gene coding for protoporphyrinogen oxidase, the molecular target of diphenyl ether-type herbicides (1996), J. Biol. Chem., 271, 9120-9128.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K424E | inactive | Saccharomyces cerevisiae |
| L422P | inactive | Saccharomyces cerevisiae |
| L422P/K424E | double mutant, inactive | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | inhibited by diphenylether herbicides | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00002 | - |
protoporphyrinogen-IX | - |
Saccharomyces cerevisiae |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59670 | - |
calculated from amino acid sequence | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protoporphyrinogen-IX + 3 O2 | Saccharomyces cerevisiae | penultimate step of heme and chlorophyll biosynthesis | protoporphyrin-IX + 3 H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.73 | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protoporphyrinogen-IX + 3 O2 | - |
Saccharomyces cerevisiae | protoporphyrin-IX + 3 H2O2 | - |
? | |
| protoporphyrinogen-IX + 3 O2 | penultimate step of heme and chlorophyll biosynthesis | Saccharomyces cerevisiae | protoporphyrin-IX + 3 H2O2 | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| flavin | - |
Saccharomyces cerevisiae | |
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