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Literature summary for 1.3.3.3 extracted from
- Purification and properties of coproporphyrinogen III oxidase from yeast (1997), Methods Enzymol., 281, 367-378.
General Stability
| General Stability | Organism |
|---|---|
| when incubated with 3 M urea, the enzyme is slowly denatured at pH 6.3 and much faster at pH 7.6 | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| coproporphyrin III | competitive inhibitor | Saccharomyces cerevisiae |  |
| N-bromosuccinimide | - |
Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
2 * 35000, SDS-PAGE | Saccharomyces cerevisiae |
| 70000 | - |
gel filtration | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coproporphyrinogen III + O2 | Saccharomyces cerevisiae | - |
coproporphyrin III + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | P11353 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Ultrogel Ac44 gel filtration | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00925 | - |
unpurified enzyme, at pH 7.6 and 25°C | Saccharomyces cerevisiae |
| 0.08483 | - |
purified enzyme, at pH 7.6 and 25°C | Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 1% (w/v) NaN3 (100 mM Tris, pH 7.6), several years, no loss of activity | Saccharomyces cerevisiae |
| 25°C, 1% (w/v) NaN3 (100 mM Tris, pH 7.6), several months, no loss of activity | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coproporphyrinogen III + O2 | - |
Saccharomyces cerevisiae | coproporphyrin III + H2O | - |
? | |
| additional information | no anaerobic activity is detected using NADP+ and S-adenosylmethionine | Saccharomyces cerevisiae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 35000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| coproporphyrinogen III oxidase | - |
Saccharomyces cerevisiae |
| CPO | - |
Saccharomyces cerevisiae |
| HEM13 | - |
Saccharomyces cerevisiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 56 | - |
a 2 minute incubation of the protein at 56°C leads to a total loss of enzymatic activity | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Saccharomyces cerevisiae |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Saccharomyces cerevisiae | isoelectric focusing | - |
6.2 |
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