Cloned (Comment) | Organism |
---|---|
gene sll1185, overexpression of N-terminally Strep-tagged HemF in Escherichia coli | Synechocystis sp. |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of enzyme-deficient DELTA sll1185 mutant, which fails to grow under aerobic conditions, with accumulation of coproporphyrin III. This growth defect is restored by cultivation under micro-oxic conditions | Synechocystis sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | is added to the assay at 10 mM | Synechocystis sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40300 | - |
x * 40300, calculated from amino acid sequence | Synechocystis sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
coproporphyrinogen III + O2 + 2 H+ | Synechocystis sp. | - |
protoporphyrinogen IX + 2 CO2 + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | - |
a single hemF gene, sll1185 | - |
Synechocystis sp. | P72848 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant terminally Strep-tagged HemF from Escherichia coli to homogeneity | Synechocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
coproporphyrinogen III + O2 + 2 H+ | - |
Synechocystis sp. | protoporphyrinogen IX + 2 CO2 + 2 H2O | - |
? | |
coproporphyrinogen III + O2 + 2 H+ | recombinant Sll1185 shows CPO activity under both aerobic and anaerobic conditions | Synechocystis sp. | protoporphyrinogen IX + 2 CO2 + 2 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40300, calculated from amino acid sequence | Synechocystis sp. |
Synonyms | Comment | Organism |
---|---|---|
coproporphyrinogen III oxidase | - |
Synechocystis sp. |
CPgen oxidase | - |
Synechocystis sp. |
CPO | - |
Synechocystis sp. |
HemF | - |
Synechocystis sp. |
Sll1185 | - |
Synechocystis sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Synechocystis sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Synechocystis sp. |
General Information | Comment | Organism |
---|---|---|
metabolism | CPO catalyzes the oxidative decarboxylation of coproporphyrinogen III to form protoporphyrinogen IX in heme biosynthesis and is shared in chlorophyll biosynthesis in photosynthetic organisms. HemF plays an essential role under aerobic growth the photosynthetic organism, overview | Synechocystis sp. |