Application | Comment | Organism |
---|---|---|
analysis | mass spectrometric assay for the two-step decarboxylative oxidation of coproporphyrinogen III to protoporphyrinogen IX catalyzed by CPO in mitochondria from human lymphocytes. The assay shows good reproducibility, uses simple workup by liquid-liquid extraction of enzymatic products, and employs commercially available substrates and internal standard. It was developed for use in clinical diagnostics of the inherited disorder hereditary coproporphyria | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000066 | - |
Coproporphyrinogen-III | pH 7.2, 37°C | Homo sapiens | |
0.000066 | - |
Coproporphyrinogen III | in 250 mM Tris-HCl, 2.5 mM EDTA, pH 7.15, at 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lymphocyte | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
coproporphyrinogen-III + ? | - |
Homo sapiens | protoporphyrinogen-IX + ? | - |
? | |
coproporphyrinogen-III + O2 + 2 H+ | CPO converts coproporphyrinogen III to protoporphyrinogen IX through harderoporphyrinogen IX | Homo sapiens | protoporphyrinogen-IX + 2 CO2 + 2 H2O | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
coproporphyrinogen III oxidase | - |
Homo sapiens |
CPO | - |
Homo sapiens |